The interaction of nucleic acid bases with the alkylating antitumour drug thiotepa has been studied by field ionization mass spectrometry. The possibility of interaction between all four DNA bases and thiotepa in neutral aqueous solutions was demonstrated. The analysis of the data for different methylated bases made it possible to elucidate some sites of alkylation-N(l)(Cyt), N(3)(Thy), N(9XAdebwhich are compared with data on other alkylating agents available from the literature.
The behaviour of 1,1',1"-phosphinothioylidinetrisaziridine (thiotepa) in water and aqueous salt solution of various acidity was studied by field ionization mass spectrometry (FI-MS), TLC and potentiometric titration. The rate of thiotepa hydrolysis increased with increasing thiotepa, or sodium chloride concentration, and with decreasing pH. As hydrolysis products of thiotepa, monoand dichloro derivatives formed in neutral, salt-containing and acidic solution, tepa, and products formed by P-N bond cleavage and release of aziridine have been identified.
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