The emergence of antibiotic-resistant bacteria is considered a serious problem. The resistance of bacteria against antimicrobial substances becomes important in the repair systems for damage to DNA and RNA molecules. The role of the antioxidant system in the development of bacterial resistance against antibiotics is not yet practically studied. The article studied the expression regulation of the genes of antioxidant enzymes and enzymes involved in the genetic information in E. coli cells with the antibiotic resistance against apramycin and cefatoxime. The study was conducted on bacterial cells resistant against these two antibiotics. The genes blaOXA-1, blaSHV, blaTEM, mdtK, aadA1, aadA2, sat, strA, blaCTX, blaPER-2, tnpA, tnpR, intC1 and intC1c were identified in bacterial cell case. This indicates the presence of plasmids in bacteria with these genes, which provide bacterial resistance to apramycin and cefatoxime. It was established that during the formation of cefotaxime resistance, there was a sharp increase in the expression of the Cu, Zn superoxide dismutase gene: in comparison with the control group, the representation of its transcripts increased 141.04 times for cefotoxime and 155.42 times for apramycin. It has been established that during the formation of resistance to the studied antibiotics in E. coli, an increase in the expression of the end4 and end3 genes is observed. There is tendency toward an increase in the number of transcripts of the pol3E gene observed in the formation of resistance against cefotaxime and apromycin.
Karachaev goat (Capra hircus) is a local breed from North-Caucasus region, Russia. Here we present complete mitochondrial genome of Karachaev goat from the republic of Karachaevo-Cherkessia, Russia. The length of the studied sequence was 16,624 bp in size. It was shown that the studied specimen belonged to haplogroup A.
The Karachaev goat is an indigenous breed that possesses unique features including significantly less fat deposition in the body compared to sheep and cattle, ability to graze at an altitude of up to 1200 meters and to produce fertile hybrids with wild relatives. To understand the genetic diversity and population structure of hybrids between domestic Karachaev goats (Capra hircus) and The West Caucasian tur (Capra caucasica) 143 individuals were analyzed using 10 microsatellites panel. Sample were analyzed according to the manufacturer’s recommendations on an automatic sequencer, ABI 3130XL genetic analyzer (Applied Biosystems). Genetic diversity was calculated using GenAlEx 6.503 software. Genotyping of ten microsatellite loci in hybrid forms of Karachaev goats and turs detected 106 alleles in total. Na values ranged from five (INR063) to seventeen (SRCRSO008), averaging 10.6 alleles per locus across the 10 loci. All loci were polymorphic. The average number of alleles was 10.6 alleles per locus. This is higher than the similar indicator obtained by Kharzinova et al. (2019) in populations of Soviet wool, Tajik wool, Orenburg downy, Alpine and Zaanen dairy breeds of goats, studied using the panel of same 10 STR-markers (9.3 loci). Other key indexes of genetic diversity could be found in table 1. The values of the coefficient FIS suggest the absence of related mating in the herd. Information on genotypic variability of Karachaev goats hybrid forms obtained here will contribute into the breeding programs improvement and to preservation of existing native breeds.
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