The connection between the separate structural parts of the mucous membrane of the small intestine of laying hens and the typological features of the autonomic tone were investigated. The studies were conducted on adult chickens of the cross-breed "Isa-Brown", which were divided into two groups: sympathicotonic chickens and sympathico-normotonic chickens, by the methods of electrocardiography and variation-pulsometry research. In the small intestine of the poultry of each group, the linear dimensions were determined first, and then the volume of the entire mucous membrane and its separate parts (villi, crypt, muscular plate), as well as the volume of connective tissue fibers in the crypt region. Research has shown that the villi account for ¾ of the volume of the entire mucous membrane. The volume of villi in the entire small intestine is more important in sympathico-normotonic chickens. This same poultry group has an advantage in the indicators of the volume of the muscular plate, but only in the duodenum and jejunum. In indicators of crypt volume, it turned out to be quite the opposite of the relationship with the typology of autonomous influences. In all studied intestines, the higher values of this indicator belong to the sympathotonic chickens. However, in terms of the volume of the entire mucous membrane, larger values still correspond to sympathico-normotonic chickens. Sympathicotonic chickens are inferior to them in the duodenum – at 1,005 mm3, in the jejunum – at 2,699 mm3 and in the ileum – only 78 mm3. Investigating the structure of the villi, we established that the volume of their epithelium in all three sections of the small intestine has higher values in the sympathico-normotonic chickens, and the larger volume of the lamina propria of the villi in the sympathicotonic chickens. At the same time, the connection with the type of autonomic tone is reflected in the ratio between the two layers of villi. In all three sections of the small intestine of sympathicotonic chickens, the ratio between the epithelium and the lamina propria was, on average, 65%/35%, or 2/1. In sympathico-normotonic chickens, this ratio varied and, on average, equalled 75%/25%, or 3/1. The increase in the tone of sympathetic centers contributes to the growth of volume both as the total amount of fibers of connective tissue, and also as separate elastic fibers in the area of crypt. According to the data,indicators of the sympathico-normotonic chickens are inferior to those of sympathicotonic chickens in all of the small intestine. It was also found that in both groups of poultry the volume of elastic fibers was approximately half the volume of all fibers of the connective tissue of the duodenum. In other sections of the small intestine, this figure was reduced to about ⅓. Consequently, the typological features of the autonomic tone, providing various trophic-regulatory effects on the mucous membrane of the small intestine, cause the formation of differences in its volume.
The tonus of autonomous centers reflected in the morpho-functional features of the organs in mammals. The study aimed to establish the influence of the autonomous regulation and its’ typological peculiarities on the structural features of the rabbits’ Harderian gland. Clinically healthy male rabbits, four months old, weighing 3.6-3.9 kg, were selected for the research. Based on the study of heart rate variability, three types of autonomous regulation were outlined, according to which three groups of rabbits were formed: ST rabbits (sympathetic dominant regulation), PS rabbits (parasympathetic dominant regulation), and NT rabbits (combined sympathetic and parasympathetic regulation). After euthanasia, the Harderian gland was dissected in all animals. Histological specimens were prepared and a morphometric examination was performed. ST rabbits corresponded to the minimal indicators in the tubular alveoli in both parts of the gland, as well as the maximum indicators of capsule thickness. NT rabbits corresponded to the maximum values of the acini area in the pink lobe, and in the white lobe - the maximal values of the cross-sectional area of the tubular alveoli, its wall area, and the epithelium height. The maximum acini area corresponded to PS rabbits in the white lobe, and in the pink lobe - the maximum indicators of the tubular alveoli cross-sectional area, and epithelial height. The pink and white lobes’ structure of the rabbit’s Harderian gland was affected by the combined tonus of the sympathetic and parasympathetic centers. The findings elucidate the regulatory and trophic effects on the Harderian gland in rabbits.
Commercial poultry production growth and the increase in the number of small farms specializing in raising broiler chickens, laying hens, quails, and other poultry contribute to the spread of infectious diseases. Non-compliance with the principles of biosafety during incubation and breeding results in mass deaths of poultry and, consequently, significant economic losses for farmers. Salmonellosis is one of the most dangerous anthropozoonotic diseases of poultry, which is most often registered in private farms. Age analysis of the poultry salmonellosis in the EU countries indicates the infection of adult laying hens most often, young poultry to a lesser extent, and chickens aged up to 10 days less frequently. Although the program for the prevention and elimination of poultry salmonellosis has been approved at the legislative level in Ukraine, monitoring studies are not carefully conducted. This is especially true for private homesteads and small farms, which greatly complicates the epizootiological situation in some regions of the country. The article describes the pathological and histological changes in the liver, heart, lungs, kidneys, and spleen. The changes were detected in chickens aged 10 and 14 days infected with microorganisms of the species Salmonella pullorum. Eggs for incubation were obtained from different family flocks, and incubation was performed in a single incubator. The initial clinical signs of the disease appeared in chickens aged 7 days and included diarrhea, increased water consumption, lameness, mass concentration of chickens near heat sources, nervous phenomena in the form of circle walking, and partial blindness in some cases. The pathological autopsy revealed hepatomegaly with sharp change in the organ colour, diapedetic hemorrhage under Glisson’s capsule, and diffuse miliary necrosis. Greyish-white nodular lesions of the lungs and heart, dystrophic changes in the kidneys, and deposition of uric acid salts in the ureters were also characteristic features. Diffuse coagulation necrosis, massive perivascular infiltration by heterophilic lymphocytes and stasis were observed in the liver. Changes in the heart were characterized by significant infiltration by mononuclear cells and heterophiles, which led to atrophy, necrosis, and replacement of cardiomyocytes by connective tissue cellular elements. Delymphatization and necrosis of the lymph nodes were pronounced in the spleen.
The article covers the results of a comprehensive pathomorphological study of Babesia canis parasitic infestation in a dog of the German boxer breed. In April 2020, a male German boxer with pronounced clinical signs of anorexia, remitting fever, hematuria, and manifestations of general jaundice was admitted to the Dr. Markevych clinic (Lviv). The anamnesis evidenced that after daily walks in the park of Lychakiv district (Lviv) the pet’s owner had been detecting 3–5 Ixodes ticks on the dog’s body for several days in a row. Cytological examination of blood smears stained by the Romanovsky–Giemsa method revealed a massive lesion of erythrocytes by the Babesia canis parasite. The applied treatment strategy, namely the introduction of the drug with the active substance imidocarb dipropionate, was not successful. The dog died in the clinic. An autopsy was performed in agreement with the pet owner. Macroscopic examination revealed foamy fluid in the trachea, pulmonary edema and lobar pneumonia, massive diffuse hemorrhages on the epicardium and costal pleura, splenomegaly, serous lymphadenitis, acute venous stasis, hepatic and renal dystrophy, and pancreonecrosis. General anemia and transudate accumulation in serous cavities were established. Tissue fragments of lungs, kidneys, liver, spleen, and pancreas were selected for a histopathological examination and fixed in 10 % neutral aqueous formalin solution, followed by dehydration in alcohols, filling into paraffin blocks and subsequent production of histopreparations. Staining was performed with eosin and Ehrlich’s hematoxylin according to the conventional methods. Microscopically, the lungs showed the alveoli swelling, thickening of the alveoli walls with blood overflow, bronchioles’ inflammatory infiltration with the accumulation of desquamated epithelium and exudate in the lumens of large bronchi. Extracapillary glomerulonephritis and hyaline-drop renal tubular dystrophy were observed in the kidneys. Vascular endothelium edema, granular hepatocyte dystrophy, sharp dilation of central vein and intraparticle and interparticle capillaries were found in the liver. Significant hyperplasia was observed in the red pulp of the spleen. Reticuloendothelial proliferation, erythrocyte proliferation with a diffuse accumulation of hemosiderophages due to increased erythrocyte disintegration, sinus dilatation, and atrophy of white pulp lymph nodes were also revealed.
Histomonosis is a fairly common disease in poultry – turkeys, laying hens, guineafowls, geese, ducks. Spontaneous cases of histomonosis in Indian peafowls do not occur very often, thus they feature less frequently in the scientific literature. Among possible reasons for this to happen is that they are kept in zoos or at private farms, where their death is mainly attributed to complications caused by E. coli or Clostridium microorganisms. The article demonstrates pathoanatomical and pathohistological changes in the organs of Indian peafowls during spontaneous histomonosis characterized by liver and caecum lesions alongside developed multifocal necrotic hepatitis and diphtheria typhlitis. Clinical signs of the disease in Indian peafowls manifested with gradual feed refusal, the bird was becoming depressed and hiding its head under the wing. The first death of a female Indian peafowl was recorded 10–14 days after the onset of such clinical signs. During histological examination, massive necrotic lesions were detected in the liver and in places of intensive development of histomonas. Cell infiltration consisting of pseudo-eosinophils, lymphoid and histiocytic cells was formed around these lesions. The penetration of histomonas into the mucous membrane of the caecum caused proliferative processes by cellular elements, which was also accompanied by heterophilic-leukocyte infiltration and the formation of cell granulomas in places where histomonas were located.
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