Background: The sternum is one of the most important and characteristic skeletal elements in birds, highly adapted for flight and showing a diverse range of morphogenesis. The Asian koel (Eudynamys scolopaceus) is a member of the Cuckoo order of birds, found in the Indian sub-continent, China and Southeast Asia. The present study was carried out as anatomical data on the sternum of this species is scanty. Methods: Carcass of Asian koel brought to the Department of Wildlife Science, Madras Veterinary College, Chennai after the post-mortem examination was biologically macerated, cleaned, dried and morphological and morphometric studies were performed. Result: Gross morphological and morphometric data of the sternum of Asian koel was compared with other avian species. The sternum of Asian koel was large, broad, quadrilateral shaped and highly pneumatized bone with well developed sternal crest, craniolateral and caudolateral processes. These features provide protection to the viscera and facilitates superior flight.
Background: Mesenchymal stem cells are well known for their self-renewal capacity and ability to differentiate into multiple cell lineages. The aim of the study was to develop a simple technique for isolation of mesenchymal stem cells from porcine adipose tissue and to study the morphometric characteristics of porcine mesenchymal stem cells.
Methods: Porcine adipose derived mesenchymal stem cells were isolated in vitro by using collagenase type II enzyme. Cell yield and viability of the cells were calculated by using trypan blue exclusion method using Neubauer’s chamber. Characterization of MSCs were done by using specific cell markers. The morphological changes, morphometry were analysed in culture using Leishman’s stain. The cell doubling (CD) and Population doubling time (PDT) were also calculated.
Result: The isolated adherent cells start forming colony and demonstrated an elongated, round and spindle like fibroblastic morphology by day 1. Almost 80-90 per cent confluency was attained on day 8-9 after the initial seeding and was reduced to day 3-4 in the subsequent passages. RT-PCR reactions revealed positive expression of mesenchymal stem cell markers CD44, CD73 and negative expression of CD34, a hematopoietic cell surface marker. Immunocytochemistry also revealed positive expression for CD44 and negative for CD34. In morphometric studies, the cell length, nucleus length, cell width and nucleus width were increased between 24 and 48 hours in both P2 and P3.
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