The feasibility of using ultrafiltration (UF) for removal of oligosaccharides from aqueous extracts of soybeans was investigated. Soybeans were soaked, blanched, ground, prefiltered, and the resulting full-fat extract processed in a hollow fiber UF unit. Initial permeate flux of 43-60 L/m'/hr at 3.5% total solids decreased semi-logarithmically to 13 L/m'/hr at 14% total solids. Low pH resulted in higher flux. Rates of removal of oligosaccharides closely followed theoretical behavior for a nonrejected solute during ultrafiltration and continuous diafiltration, and up to 96% could be removed by a twostage UF process. Final product assayed 60% protein, 35% fat and 0.6% oligosaccharides (dry basis).
Water extracts of whole soybeans were obtained as described previously (JFS 43: 354) and processed in a hollow fiber ultrafiltration unit equipped with the XM-50 membrane. Protein and fat concentration increased linearly with volume concentration ratio (VCR) as expected for rejected components, although some losses were evident probably due to membrane adsorption. Amount of ash in retentate was reduced by 55%, indicating some binding with proteins. Protein coefficients of 0.9 or better could be easily obtained. Trypsin inhibitor activity of final product was reduced by 79%, mostly due to heat treatments during extraction. Final product assayed typically 60% protein, 34% fat, 2.8% ash (dry basis) and was very low in ohgosaccharides and phytic acid.
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