Objective. To study the changes in hematological leukocyte indices among children with coronavirus infection COVID-19 in the dynamics of infectious process. Materials and methods. The work is based on the analysis of clinical and laboratory data from 91 pediatric patients with COVID-19. On the basis of a general blood test, the following hematological leukocyte indices were determined: the entropy degree of leukogram according to A.V. Gorelov et al.; leukocyte intoxication index according to V.K. Ostrovsky; nuclear endotoxicosis degree index according to G.D. Dashtayants; Harkavi adaptation index; Krebs index; leukocyte index; neutrophils to monocytes ratio index; lymphocytes to monocytes ratio index. Results. The most sensitive hematological indices were the entropy degree of leukogram according to A.V. Gorelov et al., indicating a violation of the dynamic constancy of leukogram; neutrophils to monocytes ratio indices, lymphocytes to monocytes ratio indices, showing the presence of a viral infection. At the peak of the infectious process, C-reactive blood protein index significantly correlated with the endotoxicosis index according to G.D. Dashtayants (R = 0.271; p0.05), with neutrophils to monocytes ratio index (R = 0.276; p0.05); and in the period of early convalescence - with leukocyte intoxication index according to V.K. Ostrovsky (R = 0.743; p0.05), with endotoxicosis indices according to Dashtayants (R = 0.785; p0.05) and neutrophils to monocytes ratio index (R = 0.743; p0.05). Conclusions. The calculation of hematological leukocyte indices increases the information capability of the general blood test in children with coronavirus infection COVID-19, provides important additional information on intoxication syndrome, the state of immune response, the persistence of inflammatory process during the period of convalescence as well.
Objective. Using the data obtained in Perm Region as an example, to identify the effectiveness of polymerase chain reaction (PCR) for the diagnosis of Human Monocytic Ehrlichiosis (HME) at different periods from the onset of the disease, and to determine the role of HME in the structure of infections transmitted by ixodic ticks using PCR and enzyme-linked immunosorbent assay (ELISA) Materials and methods. A thorough clinical and epidemiological examination of 583 patients with acute febrile diseases developed after the suction of ticks was carried out. To detect E. muris DNA, 1586 whole blood samples were examined by PCR at different periods from the onset of the disease. For the purpose of serological verification of HME, all patients were examined with ELISA for the presence of immunoglobulins M and G against E. chaffeensis. Results. In total, using the PCR method, ehrlichial DNA was detected in 76 (4.8 %) blood samples from 53 patients. Based on two research methods (ELISA and PCR) HME was diagnosed in 58 (9.9 %) persons, while in 50 (86.2 %) of them, the diagnosis was confirmed only by PCR. The timing of E. muris genomic material detection in the blood of patients varied from 1 to 58 days from the moment of the disease. The greatest effectiveness of PCR (up to 69.4 % of positive samples) was noted by us from the 1st to the 7th day of illness. HME was found in the form of monoinfection in 9 (15.5 %), mixed infection in 49 (84.5 %) persons. The following was revealed: HME+Ixodid tick-borne borreliosis (ITBB) in 35 (60.3 %), HME+ITBB+Human granulocytic anaplasmosis (HGA) in 6 (10.3 %), HME+ITBB+HGA+Tick-borne encephalitis (TBE) in 4 (6.9 %), HME+TBE in 2 (3.5 %), HME+TBE+ITBB in 2 (3.5 %). Conclusions. In the diagnosis of HME, PCR significantly increased the number (up to 86.2 %) of confirmed cases, and most often in the acute period of the disease (up to 69.4 15.3 % of positive samples in the first week of the disease). For laboratory verification of HME, it is advisable to combine ELISA with the PCR method, especially in case of negative results of serological studies.
and genetic peculiarities of cultural and morphological variants of Bacillus anthracis. Jurnal mikrobiologii, epidemiologii, immunobiologii. -Journal of microbiology, epidemiology and immunobiology. 2008;4:611. (In Russ.)]. 5. Sylvestre P., Couture-Tosi E. and M. Mock. Polymorphism in the collagen-like region of the Bacillus anthracis BclA protein leads to variation in exosporium filament length.
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