A study was conducted at Ido and Atiba Local Government Areas (LGAs) of Oyo State, Nigeria, to investigate the prevalence of Newcastle disease  (ND) using Enzyme Linked Immunosorbent Assay (ELISA) techniques. A total of 376 normal feathered local chickens were sampled by collecting 2 ml of blood from each bird. Sera that emanated from them were subjected to detection of ND antibodies using ELISA test kit. The data collected were analyzed by inferential statistics. The results showed that the prevalence of ND as 11.70% and 15.43% at Ido and Atiba LGAs, respectively. Adult males showed higher prevalence as compared to adult females in the two LGAs. Meanwhile the prevalence of ND in adults was higher than in the young in Ido but the reverse at Atiba LGA. Combined prevalence was averaged at 13.56% in the two LGAs. The combined results further showed that males (8.24%) were more susceptible to ND than females (5.32%) just like adults (7.45%) were more susceptible than the growers (6.11%). It can be concluded that ND is prevalent in the study areas. It can therefore be recommended that vaccination of local chickens should be vigorously  implemented since they are in the common environment/space with intensively managed birds to avert cross infection. Key words: antibodies, ELISA, local chickens, Newcastle disease, prevalence