The purpose of this search was to investigate the influence of “Thireomagnile” and “Thyrioton” on the antioxidant status of cows in the development of endotoxicosis. The experimental part of the work was performed at the Department of Pharmacology and Toxicology of Stepan Gzhytskyj Lviv National University of Veterinary Medicine and Biotechnologies, in the laboratory of Pharmacology and Toxicology of the State Scientific Research Institute of Veterinary Preparation and Feed Additives (Lviv) and in the Ltd. “Barkom”, village Yampil of Pustomyty district, Lviv region. To solve the tasks of the research, three experimental groups of 10 pregnant cows of the Ukrainian Black-Spotted Dairy breed were formed in each: control and two experimental. Cows of the control group (K) had characteristic clinical signs of endotoxicosis. Animals of the first experimental group (E1) were given 10 mL per animal of “Thyroid magnet” at the eighth and ninth months of gestation. The animals of the second experimental group (E2) were given Thyrioton at a dose of 10 mL per animal in the eighth and ninth months of gestation. As a result of the conducted researches it is found out that in pregnant cows with clinical manifestation of endotoxicosis the inhibition of the activity of the antioxidant protection system and intensification of processes of lipid peroxidation occurs. The use of “Thireomagnile” and “Thyrioton” in cows by the development of endotoxicosis contributed to the increased activity of the antioxidant system, which is indicated by an increase in the activity of glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase. The use of of “Thireomagnile” and “Thyrioton” to cows in the development of endotoxicosis contributed to the suppression of lipid peroxidation processes, which is indicated by the low level of primary and final products of lipid peroxidation, namely: lipid hydroperoxides and TBA-active products. Better normalizing action on the equilibrium on the activity of the antioxidant system and the intensity of lipid peroxidation was shown by the introduction of “Thyroid magnet” at a dose of 10 cm3 per animal.
The aim was of this study was to discover the adaptation indicators of different breeds first-calving cows to voluntary (robotic) milking system during the first month of lactation. The research was carried out in a robotic farm on German Holstein, French Holstein breeds and Brown Swiss breed of cows. During the adaptation period, the German Holsteins were differed from the rench Holsteins and Brown Swiss breed by milk yield, multiplicity of milking, and the amount of consumed concentrated feed. On the 30th day (end of adaptation period), the German Holstein breed dominated over the French Holstein breed and Brown Swiss having average daily milk yield by 0.73 and 4.12 kg, milking times by 0.26 and 0.34, the amount of consumed concentrated feed by 0.32 and 0.61 kg higher. In addition, the German Holstein breed during the adaptation period was distinguished by higher multiplicity of passages through the selection gate and the number of visits to feed stations. Important indicators of adaptation to free keeping and voluntary milking are the number of cases of forced milking. On the 5th day of lactation, some 69.4–86.2% of cows of all the groups did not enter the milking robot. This indicator has been steadily declining every five days. On the 30th day of lactation the cases of operator-forced milking were 24.3–35.9% and they were the lowest in cows of the German Holstein breed, indicating the best adaptive qualities of this cattle. The German Holstein breed had more lower (by 0.30 and 0.26 mS/cm) electrical milk conductivity on the 30th day compared with French and Brown Swiss breeds. In addition, the number of somatic cells in its milk was lower by 19.4 and 17.1 thousand cells per cm3 compared with French Holstein and Brown Swiss breed. Therefore, we suggested that the German Holstein breed has more higher stress tolerance, and hence a shorter adaptation time to the keeping.
From the standpoint of modern ideas, the normal functioning of the poultry body and full implementation her of the genetic potential is impossible without the presence of selenium in the diet. The biochemical diversity of selenium puts it in a number of priority trace elements. Scientists who were studying the effects of selenium on poultry body and paid relatively little attention to the quality of the meat. In scientific experiment are studied the effect of additives different doses of selenium in compound feed on the chemical composition, energy and biological value of meat of the cross chickens-broilers COBB 500. Introduction of selenium into the feed for broiler chickens in the doses which are studied (0.3 mg/kg, 0.4 and 0.5 mg/kg) did not significantly affect to the quality of their muscle tissue although it positively affected on some indicators that characterizing its chemical composition, nutritional and biological value. Among the experimental groups, the best meat quality indicators were in the second group of chickens for which was introduced into the feed selenium from the calculation of 0.3 mg/kg. When feeding mixed feeds with selenium additives to broiler chickens it was found that it is likely to increase the concentration of this trace element in muscle tissue by 60.6-100% (P?0.001), which does not exceed the maximum permissible level (MPL). Consumption of selenium-enriched meat of broiler chickens within the limits of the physiological norms recommended in Ukraine will ensure the daily requirement of an adult in this trace element by 23.6-29.4%. This meat product can be especially useful for people who live in regions with low levels of selenium in the natural environment.
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