Hypericin was isolated from Diahyperon tincture for use as a standard sample. The chromatographic purity of the hypericin was confi rmed by high-performance liquid chromatography/mass spectrometry. Hypericin has the only chromatographic peak with a retention time of 23.10 min and gave a characteristic signal for the molecular ion [M -H + ]with m/z 504.05 in the mass spectrum. Electronic absorption spectra of Diahyperon tincture and the MeOH extract of Hyperici herba (Hypericum perforatum) showed characteristic hypericin bands at 550 and 590 nm and a band at 665 nm that was due to the presence of chlorophyll. It was shown that excitation of hypericin in Diahyperon tincture at λ ex = 470 nm gave an emission spectrum with characteristic hypericin bands at 593 and 640 nm while the emission spectrum of the MeOH extract of Hyperici herba showed the hypericin emission bands and a weak chlorophyll emission band at 670 nm. It was found that spectrophotometric and spectrofl uorimetric determinations of hypericin in Diahyperon tincture and in Hyperici herba gave similar results that were consistent with the standardized values of the hypericin content in the studied samples.
Industrial manufacture of palleted medicinal preparations requires the use of excipients of various purposes among which magnesium stearate is of great importance giving lubrication properties to the tabletted substance. However, magnesium stearate has an unequal lubricating effect despite the fact that quality indicators of magnesium stearate from different manufacturers comply with the requirements of the Pharmacopoeia Monograph. In this work, a comparative analysis of magnesium stearate quality indicators from Merck KGaA (Germany) and Accent Microcell Pvt. Ltd. (India) was carried out. It was found that magnesium stearate from Merck KGaA (Germany) has the best lubrication properties having the smallest particle size and predominantly dihydrated form and characterized by the highest content of stearic acid. To identify hydrated forms of magnesium stearate thermal analysis was used making it possible to determine dehydration temperatures and melting of pseudopolymorphic structures by endothermic heat effects.
When obtaining human albumin medicinal preparations with a high binding capacity to be used in detoxification therapy, an urgent task is to control residual content of bilirubin. Hence the purpose of this investigation is to study the possibility of applying derivative spectrophotometry for bilirubin assay in human albumin medicinal preparations. Record of the first-order spectra in combination with the spike test made it possible to obtain a linear dependence of the value |dA/dλ| in the minimum of the absorption band at a wavelength of 516 nm from the concentration of added bilirubin and to determine statistically significant bilirubin concentration in the medicinal preparation "Albupharm, solution for infusions, 200mg/ml" (JV Pharmland LLC, Republic of Belarus), which was 8,58 ± 0,23 μM, and also to draw a conclusion about the efficiency of purification technology of albumin from bilirubin.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.