Com par i son of gene ex pres sion pro files in hu man nor mal brain and glioblastoma us ing SAGE da ta base revealed 129 genes with 5-fold dif fer ence of ex pres sion level in glioblastoma (P£0.05), 85 of them were down-reg u lated. The num ber of genes with 5-fold down-reg u lated ex pres sion is less in the dif fuse and anaplastic astrocytomas. Five-fold de crease of the ex pres sion in the dif fuse astrocytoma and nearly the same ex pres sion lev els in the anaplastic astrocytoma and glioblastoma were re vealed for 9 genes only. For over whelm ing ma jor ity of in ac ti vated genes in the low-grade astrocytoma the ex pres sion level de creased pro gres sively in the sub se quent stages of ma lig nant pro gres sion of astrocytoma. Ex pres sion lev els of some genes were very low or un de tect able in glioblastoma, the most ag gres sive brain tu mour. The de creased expres sion of se lected genes in glioblastoma was con firmed by North ern anal y sis and RT-PCR. Some genes, de scribed in this work, may en code the tu mour suppressors and their de creased ex pres sion may play an impor tant role in ini ti a tion and pro gres sion of hu man glioma.
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