867Acinetobacter lwofi, Azotobacter vinelandii, Agrobacterium and Rhizobium species contain quinoprotein glucose dehydrogenase apoenzyme (EC 1.1.99.17). Addition to whole cells of pyrrolo-quinoline quinone (PQQ), the prosthetic group of this enzyme, resulted in the production of gluconic acid from glucose. The in vivo reconstitution of apo-glucose dehydrogenase with PQQ was dependent on the presence of Ca2+ or Mg2+. Optimal conditions for reconstitution allowed maximal glucose dehydrogenase activity in the presence of 1-1 0 nmol PQQ 1-l. Synthesis of the apoenzyme of glucose dehydrogenase was not dependent on glucose in the growth media. The physiological significance of the synthesis of apo-glucose dehydrogenase, as found in a variety of bacteria, is discussed.
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