Seed-born fungi of wheat interact with the plant at various stages of its development and with each other. With the highest growth rate, they should be isolated because of competition. The purpose of this study was to compare the growth of colonies on a nutrient medium for the gradation of fungal genera and species from wheat seeds according to aggressiveness. These data helped concluding on the effectiveness of mycoexpertise of winter wheat seeds. Potatoglucose agar (PGA) was used for the analysis of the fungal complex. Seven-day fungal cultures were sown in the centre of Petri dishes. Linear growth of fungal colonies on PGA with gentamicin was determined. The specific features of the development of 12 representatives of seeds mycobiota from the Northeast of Ukraine of the 2017-2019 harvest were investigated. The dominance of Alternaria sp. and a slight release of Fusarium sp. were established by analysis of the fungal complex. The first comparison of the linear growth of Fusarium graminearum, F. poae, and Alternaria tenuissima in 2017 showed that Fusarium colonies grow faster on nutrient medium. In 2018, the growth characteristics of A. arborescens, which quickly became dominant in wheat seeds mycoflora, and the littlecommon Trichothecium roseum were studied in detail. By comparing the growth of fast-growing F. graminearum with the common Aureobasidium pullulans and the aggressive Nigrospora oryzae, the fastest development of the third and the slowest of the second species was established. F. poae filled the Petri dish on day 6, Penicillium – on day 22. In 2019, in the first experiment comparing F. poae, F. sporotrichioides, and A. avenicola, the second species had the worst growth rates. It became the second fastest growing colony in the study of the growth of seven species in the second experiment. Isolates of N. oryzae in 2018 were more aggressive than in 2019. Specific features of colony growth on PGA did not affect the effectiveness of the analysis of mycobiota of winter wheat seeds. N. oryzae had the highest radial speed under the general dominance of Alternaria sp. Fusarium sp. (F. poae, F. sporotrichioides, F. verticillioides, and F. graminearum) and B. sorokiniana developed rapidly. A. arborescens and A. avenicola grew at the same level as A. pullulans. Penicillium and T. roseum lagged behind other fungi in speed and filled Petri dishes for the longest time. A. tenuissima had the lowest radial growth rate
Goal. To investigate the fungistatic effect of plant extracts on Alternaria tenuissima in vitro. Methods. The study was conducted in the Laboratory of Phytopathology of the Institute of Plant Protection of NAAS (IPP NAAS) and the Laboratory of Ecology and Pharmacognosy of Research Station of Medicinal Plants of the Institute of Agroecology and Environmental Management NAAS (RSMP IAEM NAAS). Medicinal plant raw materials used in the research were grown and selected at the research sites of DSLR IAP NAAS. Plant extracts were made on its basis. Determination of the activity of plant extracts on the growth of Alternaria tenuissima culture was performed in the laboratory of phytopathology of the IPP NAAS. Agar-disk diffusion method was used. The radial growth rate and the percentage of growth inhibition of colonies were determined. Results. On the 5th day after the start of the experiment, all studied extracts formed colonies of significantly smaller size compared to the control. On the 7th day, extracts of sage, thyme, annual wormwood, wormwood, echinacea root, and plume poppy significantly inhibited the development of Alternaria tenuissima colonies. On the 10th day, a significant reduction in the growth of colonies of the pathogen occurred with the use of extracts of sage, annual wormwood, echinacea roots and plume poppy. Inhibition of colony growth was highest for sage, annual wormwood and plume poppy and ranged from 84.3—99.5% on day 5 to 38.1—73.4% on day 10 after inoculation. Conclusions. According to our results, extracts of sage (Salvia officinalis L.), annual wormwood (Artemisia annua L.) and plume poppy (Macleaya cordata L.) showed a pronounced fungistatic effect against Alternaria tenuissima. These data suggest that extracts of these plants can be used in the future to develop plant protection products.
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