In man-Chinese hamster somatic cell hybrids the segregation of the loci for 27 human enzyme markers and the species-specific surface antigens, including the HL-A histocompatibility antigens, was studied. The results show a synteny of the human loci for phosphoglucomutase 3, cytoplasmic malic enzyme, tetrameric indophenol oxidase, and HL-A. Furthermore, evidence is presented that the loci for the human species-specific antigens are distributed over several chromosomes.Human chromosomes are lost preferentially in man-rodent somatic cell hybrids. The study of these hybrids has considerably facilitated the analysis of gene linkage in man (1). Two or more human markers are located on the same chromosome (syntenic) when they are present or absent simultaneously in hybrid cell populations. M\ost of the known human syntenic groups detected via the analysis of hybrid cells are connected with loci coding for enzymes, since most of the homologous enzymes of man and rodent origin can be distinguished by means of electrophoretic techniques. Besides enzymes, antigens of both parental genomes are expressed in the hybrid cells (2). The genes coding for antigens are therefore another class of markers which can be used in synteny studies, as has been shown by Puck (3).In the present investigation the segregation patterns of 27 loci coding for human enzymes were studied and related to the expression of human species-specific surface antigens in manChinese hamster somatic cell hybrids. Furthermore, we examined whether the human locus for HL-A was segregating in close association with the locus for phosphoglucomutase 3 (PGM3, EC 2.7.5.1) in these hybrid cells, since family studies have suggested that these loci are linked (4, 5).
MATERIALS AND METHODSHybrid somatic cell lines were obtained by fusion of mutant cell lines derived from the Chinese hamster DON cell line with normal or hypoxanthine-guanine phosphoribosyltransAbbreviations: HL-A, human histocompatibility antigens; PGM3, phosphoglucomutase 3; ME,, cytoplasmic malic enzyme; IPO-B, tetrameric indophenol oxidase; Ra/HeLa, rabbit antiserum against HeLa cells; ALS, horse anti-human lymphocyte serum; Hex-B, hexosaminidase B.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.