Hyperbranched pine-like gold nanostructure was electrodeposited on the polycrystalline gold surface at 0 mV (vs. AgCl) with the assistance of histidine as a soft template. The nanostructure was then applied as a highly sensitive nonenzymatic sensor for glucose. The catalytic activity and sensitivity of the gold nanostructure toward the electrooxidation of glucose was excellent without surface fouling and deterioration effects. The current related to the oxidation of glucose rapidly and linearly depended on its concentration with a sensitivity of 776.8 μA cm(-2)mmol(-1)dm(3), a detection limit of 3.39 μmol dm(-3) with a relative standard deviation of 2.32%.