This study critically analyzes the recent trend of Englishing shop names in Aceh province. Borrowing Bhabha’s postcolonial theory, this study attempted to uncover the shop owners’ perceptions of privileging English and marginalizing local languages, even though their customers are mostly local. Data collection was by taking pictures and interviewing 20 owners of the shops, four females and 16 males. Results show that the 120 shop names in Banda Aceh and surrounding it are in English, modified English and Indonesian ordering, hybridized English and Indonesian ordering, hybridized English Acehnese language and English, English and English ordering, and mixed owner’s names and English ordering. They voluntarily mimicked the Western culture by using English names because they believe that English is marketable, modern, practical, flexible, familiar, and short for shop branding, while the local language looks not modern, unpractical, and weird. In conclusion, most shop owners in the two districts in Aceh negotiate the Western hegemony for the economic benefits.
Multiplex-PCR (mPCR) is a technique that is gaining popularity due to its ability to detect multiple species in a single tube, resulting in faster results with more efficient and cost-effective resources than the conventional PCR method. This study aimed to design mPCR genetic markers for the detection of bovine/rat/chicken/pork organisms in processed foods. The procedure was divided into two stages: in silico and in vitro. The primers were designed based on mitochondrial cytochrome genes obtained from the NCBI GeneBank. Following alignment, the selected DNA fragments were used as the target sequence for the primer design. The sequences of all primer pairs were aligned on the Oli2go primary pooler to determine whether there was a possibility of cross reaction. The results of this study indicated that primers designed for the COX1 gene in rats and bovine, the KEF22 r01 gene in pork, and the ND6 gene in chicken produced amplicons with sizes of 622 bp, 552 bp, 588 bp, and 272 bp, respectively. However, the amplicons generated from pork, bovine and mouse marker primers were difficult to distinguish when electrophoresed in the same 2% agarose gel. Meanwhile, the amplicons of the primer markers of chickens can be distinguished when electrophoresed with pork/cows/rat. The study concludes that the designed primer pairs can be used together in mPCR if the difference in amplicon size is greater than 200 bp.Key words: Multiplex PCR; DNA markers; Halal meat test.ABSTRAKMultiplex-PCR merupakan metode yang saat ini sedang populer untuk digunakan karena dapat melakukan pendeteksian lebih dari satu spesies dalam satu tabung, sehingga hasil yang diperoleh lebih cepat, efisien, dan murah dibanding metode PCR biasa. Penelitian ini bertujuan untuk merancang penanda genetik multipleks-PCR untuk deteksi adanya organisme sapi/tikus/ayam/babi pada makanan olahan. Metode yang digunakan terdiri dari dua tahap, yaitu secara in silico dan in vitro. Primer dirancang dengan menggunakan gen sitokrom mitokondria yang didapat dari GeneBank NCBI. Setelah dilakukan alignment, fragmen DNA yang terpilih dijadikan sebagai target sekuen untuk desain primer. Semua sikuen pasangan primer di-alignment pada Oli2go primer pooler untuk melihat kemungkinan adanya cross reaction. Hasil dari penelitian ini yaitu primer tikus dan sapi dirancang dengan menggunakan gen COX1, primer babi menggunakan gen KEF22_r01, dan primer ayam menggunakan gen ND6 menghasilkan amplikon dengan ukuran berturut-turut 622 pb, 552 pb, 588 pb dan 272 pb. Namun, amplikon yang dihasilkan dari primer penanda babi, sapi dan tikus sulit dibedakan saat dielektroforesis dalam sumur gel agaros 2% yang sama. Sedangkan amplikon dari pasangan primer penanda ayam dapat terbedakan saat dielektroforesis baik bersama babi/sapi/tikus. Kesimpulan dari penelitian ini adalah hasil rancangan primer penanda telah dapat digunakan secara bersama jika perbedaan ukuran amplikon >200 pb.Kata kunci: Multiplex PCR; Penanda DNA; Uji halal daging.
<p>Tujuan dari penelitian Turbin Angin Poros Horizontal Double Multiflat Blade adalah membandingkan kinerja turbin angin poros horizonal single multiflat blade dengan turbin angin poros horizontal double multiflat blade perlakuan sisi masuk dan perlakuan sisi keluar. Pengujian turbin angin single multiflat blade dilakukan pada kecepatan angin 5 m/s, 7 m/s, dan 9 m/s dengan sudut blade 35°, 38°,40°, 43° dan 45°. Berdasarkan pengujian, turbin angin single multiflat blade pada kecepatan angin 9 m/s dan sudut blade 40° menghasilkan efisiensi sistem tertinggi yaitu 8,397%. Kecepatan dan sudut tersebut digunakan untuk pengujian turbin angin double multiflat blade. Pengujian turbin angin double multiflat blade dengan perlakuan sisi keluar, efisiensi sistem tertinggi yaitu 11,74%, sedangkan pengujian turbin angin double multiflat blade dengan perlakuan sisi masuk, efisiensi sistem tertinggi yaitu 5,16%. Kesimpulan dari pengujian adalah efisiensi sistem tertinggi dihasilkan pada pengujian turbin angin poros horizontal double multiflat blade perlakuan pada sisi keluar dengan kecepatan angin 9 m/s dan sudut blade 40° sebesar11,74%.</p>
This study aims to identify and understand the writing of fiction from the perspective of Maqāṣid al-Syarī`ah. This research uses descriptive-analytic research with qualitative (non-statistical) data collection methods, which focus on the study of texts and texts based on the juridical-normative approach. The results of the study indicate that the writing of fiction in the perspective of Maqāṣid al-Syarī`ah is permissible, subject to two conditions: first, the author states implicitly or explicitly that the story is imaginary; second, the content of fictional stories does not conflict with the teachings of Islam, but invites goodness.
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