Objective: To investigate the cellular and molecular epidemiology of acute leukemias in vulnerable populations of children and adolescents in Oaxaca de Juarez, Mexico. Material and Methods: Descriptive, cross-sectional and retrospective study, conducted from 2014 to 2018 in which profiles of molecular and immunophenotypic aberrations were investigated in children and adolescents diagnosed with acute leukemia, by evaluating 28 molecular abnormalities by HemaVision-Q28 multiplex RT-PCR kit and standardized EuroFlow Immunophenotyping of bone marrow cells. Results: We included 218 patients, with 82.5% younger than 14 years and 17.5% adolescents. The median age was 9 years and a main peak of incidence was recorded at age of 4 to 5 years. B-cell acute lymphoblastic leukemia was diagnosed in 70.64% of all cases, acute myeloid leukemia was in 22.48%, T-cell acute lymphoblastic leukemia in 6.42%, and mixed lineage acute leukemia in 0.46% of cases. Overall, chromosomal translocations were positive in 29.82% of cases. While 65.31% of patients with acute myeloid leukemia reported aberrancies, only in 18.83% of B-cell acute lymphoblastic leukemia cases genetic abnormalities were obvious. Surprisingly, most prevalent translocations in B-cell acute lymphoblastic leukemia were t(9;22) in 20.7%, followed by t(4;11) in 17.2% and t(6;11) in 13.8%, whereas patients with acute myeloid leukemia showed t(15;17) in 40.6% and t(8;21) in 21.9%. In contrast, an homogeneous expression of t(3;21) and t(6;11) was recorded for T-cell acute lymphoblastic leukemia and mixed lineage acute leukemia cases, respectively. Except for t(1;19), expressed only by pre-B cells, there was no association of any of the studied translocations with differentiation stages of the B-leukemic developmental pathway. Conclusion: Our findings identify near 50% of patients with acute lymphoblastic leukemia at debut with high-risk translocations and poor prognosis in B-cell acute lymphoblastic leukemia as well as an unexpected increase of acute myeloid leukemia cases in young children, suggesting a molecular shift that support a higher incidence of poor prognosis cases in Oaxaca.

Acute leukemia is the leading cause of death in children worldwide, particularly in developing countries where the growing number of cases with unfavorable prognosis and high risk of early relapse have positioned pediatric cancer as a priority. The late and imprecise diagnosis, malnutrition and unfavorable environmental conditions, and toxicity-associated therapy are some of the factors that compromise the success of the treatment and affect survival rates in vulnerable regions. An early and exhaustive classification of malignant neoplasms at the clinical debut and the proper follow-up of treatment's response constitute one of the most powerful prognostic factors. Remarkably, the ultrasensitive detection of residual and relapse clones that determine the minimal/measurable residual disease (MRD) has been a milestone in the comprehensive management of hematologic malignancies that favorably improve the complete remission cases. In this review, we discuss the scientific and technological advances applied to laboratory diagnosis in MRD determination: from the multiparametric immunophenotyping to next-generation sequencing and cytomics. As a result of multidisciplinary research in the main concentration oncology centers and laboratories, residual leukemia detection strategies that combine molecular analysis and cellular markers are recommended as the most valuable tools, making them the paradigm for stratification campaigns in vulnerable regions.

Introducción.En México, la mayor parte de las investigaciones han sido enfocadas a la percepción de la imagen corporal en la población urbana y existe un déficit importante de investigaciónen esta área, en población rural y/o indígena. Objetivo.Asociar la percepción de la imagen corporal con el estado nutricio y emocional en adolescentes indígenas de la comunidad zapoteca de Coatecas Altas, Oaxaca. Material y métodos.Se determinó el índice de masa corporal, y para la percepción de la imagen corporal se aplicaron encuestas autoadministradas. La parte emocional fue cuantificada de acuerdo a la escala de Rosenberg sobre la autoestima y la de Goldberg para ansiedad y depresión. Finalmente, se realizó una regresión logística binaria para identificar las asociaciones correspondientes. Resultados.El 12.19% de la población de estudio presentó bajo peso y 16.45% exceso de peso, el 29.25% tuvo una percepción de la imagen corporal que no correspondía con la realidad. El 84.7% presentó autoestima baja, 23.78% ansiedad y 5.48% depresión y  la percepción de la imagen corporal se asoció al estado nutricional (p= 0.008). Conclusiones.Se identificó que la población adolescente indígena al igual que la de zonas urbanas se asocia la PIC al estado nutricional de acuerdo al IMC. Sin embargo, no se encontró asociación entre la PIC y la parte emocional. Pero resulta importante realizar más investigación para determinar porque en zonas de muy alta marginación no se tuvo esta tendencia.

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