Exposure to acrylamide (Ac) through food is almost inevitable and this kind of toxicity may cause lifelong harm. In present study, we researched effects of Crocin (Cr) on testis histopathology in Ac‐induced testis of rats. Adult male rats were grouped as: group 1, 1 ml saline only; group 2, 50 mg/kg Cr only; group 3, 25 mg/kg Ac only and group 4, 25 mg/kg Ac + 50 mg/kg Cr. All administrations were given as 1 ml/day by gavage for 21 days. It was found that Ac adversely influenced the levels of FSH, testosterone and LH in the blood serum; malondialdehyde (MDA), total antioxidant status (TOS), oxidative stress index (OSI)/ glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), total antioxidant status (TAS) oxidant/antioxidant parameters in testis tissue (p < .01) and the histopathological parameters like Johnson's score, seminiferous tubule diameter, seminiferous epithelial height and H‐score for caspase‐3 immunoreactivity. In contrary, Cr treatment resulted in increase in testosterone, follicle stimulating hormone (FSH), luteinizan hormone (LH) levels and SOD, CAT, GSH, TAS levels (p < .01) and improved all the histopathological changes. In conclusion, Cr has a promising protective potential against Ac‐caused toxic damages in testicular tissue.
BACKGROUND:Duodenal ulcer perforation is a serious condition. A number of methods have been defined and used in surgical treatment. In this study, it was aimed to compare the effectiveness of "primary repair" and "drain placement without repair" methods in duodenal perforations using an animal model. METHODS:Three equivalent groups of ten rats each were formed. Perforation was created in the duodenum in the first (primary repair/sutured group) and the second group (drain placement without repair/sutureless drainage group). In the first group, the perforation was repaired with sutures. In the second group, only a drain was placed in the abdomen without sutures. In the third group (control group), only laparotomy was performed. Neutrophil count, sedimentation, serum C-reactive protein (CRP), serum total antioxidant capacity (TAC), serum total thiol, serum native thiol, and serum myeloperoxidase (MPO) analyses were performed on animal subjects in the pre-operative period and on the post-operative 1 st and 7 th days. Histological and immunohistochemical (transforming growth factor-beta 1 [TGF-β1]) analyzes were performed. Blood analysis, histological, and immunohistochemical findings obtained from the groups were compared statistically. RESULTS:There was no significant difference between the first and second groups, except for the TAC on the post-operative 7 th day and MPO values on the post-operative 1 st day (P>0.05). Although tissue healing was more pronounced in the second group than in the first group, there was no significant difference between the groups (P>0.05). TGF-β1 immunoreactivity observed in the second group was found to be significantly higher than in the first group (P<0.05). CONCLUSION:We think that the sutureless drainage method is as effective as the primary repair method in the treatment of duodenal ulcer perforation and can be safely applied as an alternative to the primary repair method. However, further studies are needed to fully determine the efficacy of the sutureless drainage method.
The current study aimed to observe the toxic effects of Tartrazine, a commonly used dyestuff in industries and foods, on the liver, and investigate whether this toxicity could be eliminated with Thymoquinone coadministration. The study was conducted on 32 200-250 g male Wistar albino rats, procured from İnönü University Experimental Animals Breeding and Research Center. The rats were randomly assigned to 4 equal groups: Control group, Thymoquinone group, Tartrazine group, Thymoquinone + Tartrazine group. The trials were conducted for 21 days. Then, rat liver tissue and blood samples were obtained and biochemical and histopthological examinations were conducted on the samples. Tartrazine administration increased the oxidant and oxidative stress index parameters in the liver tissue, decreased the antioxidant parameters, led to histopathological problems and inflammation in the serum samples. Thymoquinone administration increased the antioxidant parameter levels in liver tissue and decreased serum inflammation levels (p<0.001). Thymoquinone and Tartrazine coadministration reduced the adverse biochemical and histopathological effects of Tartrazine. Tartrazine caused hepatotoxicity via cellular damage in the liver tissue and led to inflammation. Thymoquinone, on the other hand, improved antioxidant and anti-inflammatory effects. We recommend daily consumption of black cumin and its active ingredient, Thymoquinone to reduce Tartrazine toxicity.
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