A total of 251 Dura cross Pisifera (DxP) hybrid palms from six populations descending from six parental African Pisifera origins and involving 12 progenies were analyzed with 19 selected Simple Sequence Repeats (SSR) markers. A total of 110 alleles were produced, ranging from three to eight per SSR, with a mean of 5.8 alleles per SSR locus. Of these, 68.5% were considered shared alleles by more than one population and the remaining 31.5% were population specific alleles. They generated between six and 21 haplotypes in all populations, and depending on the SSR marker, between one and 10 haplotypes within populations. Various parameters for analyzing genetic variability, differentiation and genetic structure were computed using GenAlEx, Structure and Darwin software. The obtained results confirmed microsatellites as a robust, feasible and trustful method for obtaining DNA fingerprints, tracing the source of oil palm samples. With respect to the authenticity of materials or for solving legitimacy issues, accession belonging to each population by SSR markers could be distinguished, but additional SSR should be screened for improving this process.
Pratama et al, 2018. Oil Palm Breeding Program Strategy for Suboptimal Area in PT Binasawit Makmur . JLSO 7(1): Suboptimal areas have great potential to be used an alternative land for cultivation of oil palm.We needed superior oil palm material to be able to grow well on that land. PT. Binasawit Makmur (BSM) through the Breeding Unit has developed a strategy regarding the assembly of superior material oil palm drought resistant. Therefore, this research aimed to develop a strategy regarding breeding program in suboptimal lands of PT Binasawit Makmur. The strategies used were introducing, evaluating, and selecting genetic material based on productivity components (total and weight of fresh fruit bunches/ha, extraction rate) and high increment. In addition, the efforts have been made to obtain drought tolerant material are conducting a progeny trial (DXP Trial) on mature palm to obtain drought tolerant in the dried season. Based on the results of the progeny trial, both of parents were traced to a crossing program. Currently, the seeds of the crossing have been obtained to confirm the results of the research on the nursery scale. In the nursery trial, research will be conducted to measure the proline content, stomata and chlorophyll content. Selection in the mature palm DXP progeny trial was followed by application a molecular tool such as SSR and SNP. Through this approach, a superior genetic material in terms of productivity and also drought tolerance can be developed and therefore can be used for recombination and release of new varieties.
Sarimana U, Indarto N, Herrero J, Erika P, Wendra F, Zulhermana Z, Asmono D. 2019. Genetic diversity preliminary study of cameroon origin oil palm at PT. Binasawit Makmur. Jurnal Lahan Suboptimal: Journal of Suboptimal Lands. 8(2):126-133. A plant breeding program needs genetic diversity information of germplasm, but the source of genetic diversity of oil palm plants in Indonesia is very narrow. In order to increase genetic diversity, the introduction of germplasm from Cameroon was conducted. The purpose of this study was to obtain a useful molecular marker in order to get genetic diversity information in Elaeis guinensis germplasm materials from Cameroon at PT. Binasawit Makmur (BSM). This preliminary research has been done in 2013. In this study, 300 samples of Cameroon oil palm DNA from 99 accessions and 10 samples DNA BSM material were analyzed by 9 microsatellite markers (simple sequence repeat; SSR). The 9 SSR markers used were very informative and we obtained 93 alleles with an average of 10.33 alleles per locus. The value of polymorphism information content (PIC) was around 0.746-0.889. The total genetic diversity was relative high (HT = 0.814). The coordinate analysis showed no groups in both 99 accessions and BSM material. Based on phylogenetic analysis, 9 SSR markers could show the genetic diversity between the populations. To validate this fact, it needs further research using more molecular markers and samples.
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