Beauveria bassiana (Bals.) Vuill is one of entomopathogenic fungus, that be able to endophytically colonize different plants. Beauveria bassiana can promote the growth of red chili following their endophytic establishment within plants through seed treatment, soil drenching and foliar spraying. The aims of this study were: (i) To get the best colonisation B. bassiana from different isolates and to differentiate inoculation methods, i.e. seed immersion, soil drenching and foliar spraying, for the endophytic inoculation of B. bassiana in red chili; and (ii) to assess the effect of B.bassiana to stimulate or trigger plant growth from isolate Wheat, Coffea, Cacao and Leptocorisa acuta. Our result showed that all of B. bassiana isolate were able to colonize seedling of chili and B.bassiana of wheat isolate was the best isolate giving spuring seedling or plant growth. From the three inoculation methodes, inoculation B. bassiana by foliar spraying was the highest colonisation. The highest colonization was in leaves, and the second were in stem. Root plant was the poorly colonized by B. bassiana fungus.

Abstract. Rahma H, Nurbailis, Kristina N. 2019. Characterization and potential of plant growth-promoting rhizobacteria on rice seedling growth and the effect on Xanthomonas oryzae pv. oryzae. Biodiversitas 20: 3654-3661. Xanthomonas oryzae pv. oryzae (Xoo), a major limiting factor in rice production, and the use of resistant Xoo varieties have failed to control the bacterial pathogens as well as increased yield. It is due to the diversity in pathotypes, caused by environmental factors, the nature of resistant variety used, and gene mutation. The aims of this study were to select rhizobacterial strains with the potential of suppressing Xoo growth and promoting the growth of rice seedlings. This experiment was conducted in a completely randomized design (CRD) using seven rhizobacterial isolates selected through a dual culture test, with four replications. There were four isolates that potential in inhibiting the growth of Xoo, namely KJKB5.4, LMTSA5.4, Bacillus cereus AJ34, and Alcaligenes faecalis AJ14, with inhibition diameters greater than 11.50 mm. Rhizobacterial supernatant of 4 potential isolates has a zone of inhibition ranging from 12.25 to 24.00 mm. Four potential isolates were also able to solubilize phosphate, produce indole acetic acid (IAA) growth hormone, and siderophore, as well as enhance the growth of rice seedlings. Based on the nucleic acid sequencing of LMTSA5.4, KJKB5.4, and RK12 isolates were identified as Stenotrophomonas malthopilia strain LMG 958 (99.13%) accession NR 119220.1, Stenotrophomonas pavanii strain LMG 25348 (95.84%) accession NR 118008.1 and Ochrobactrum ciceri strain ca-34 (92.91%) accession NR115819.1.

The aims of the research were to determine the best organic matter as carrier for growth and increasing the density of Trichoderma viride strain TV-T1sk (TV-T1sk) and its influence on Fusarium wilt disease development on banana seedling.  This research consisted of two experiments, i.e. (1). Growth  testing of TV-T1sk  in various organic matter, (2). Various organic matter testing for increasing the density of TV-T1sk in banana rhizosphere and its influence on  Fusarium wilt desease development.  The treatments were various organic matter, namely rice straw, sugar cane pulp, rice bran+banana plant waste,  hull of rice+sugar cane pulp and rice. The variables observed were  density and viability of conidia on various organic matter. Propagule density after introduction to banana rhizosphere was determined by observing TV-T1sk propagul from the rizosphere of banana seedling. The influence of density on Fusarium wilt disease development was determined by incubation period, the percentage of symptomized leaves and the intensity of corm damage. The results showed that both sugar cane pulp and rice were the best organic matter  for increasing  the growth and the density  of TV-T1sk  after introduction to banana rhizosphere. The density of TV-T1sk propagule on banana rhizophere influenced  the development of Fusarium wilt disease.

Rhizobacteria is an environmentally friendly and sustainable biocontrol agent, used as an alternative in the management of plant pathogens. Furthermore, their suppression mechanism often varies, as some are capable of producing antibiotic compounds, competing with, and increasing plant growth, although t some forms possess multiple control. This study, therefore, aims to determine the activity of rhizobacterial antibiosis against Curvularia lunata in vitro. The experiment was conducted at the Laboratory of Biological Control, Department of Pests and Plant Diseases, Faculty of Andalas University. In addition, the procedure required using an experimental method, which was carried out in a Completely Randomized Design. The antagonistic rhizobacterial selection of C. lunata was carried out using a dual culture test, on a total of 7 rhizobacteria, and 4 were selected, encompassing Stenotrophomonas malthopilia KJKB5.4, Stenotrophomonas pavanii LMTSA5.4, Bacillus cereus AJ34 and Alcaligenes faecalis AJ14 as potential candidates in the control of C. lunata, suppression effectiveness that was above 50%. In addition, it was also established that all four isolates the propensity to produce chitinase enzymes.

Endophytic fungi in lately was getting a lot of attention in agriculture. Besides being able to increase plant resistance against plant pest organism, it has also the ability to stimulate germination and pant growth. One of endophytic fungi tested in its research was Beauveria bassiana from isolate Wheat, Coffeae, Cacao and entomopatogen fungus from Leptocorisa acuta insect. The aim of this research was to know the ability of Beauveria bassiana from some tested isolates and its effect  on chlorophyll content of leaf chili  followed Mack Kinner methode by doing seed treatment aplication. Colonization and chlorophyl content of chili leaf was done on 7 MPI. The research design used Random Block Design non factorial, data was analysis by 8 STAT. The result showed all of Beauveria bassiana isolates were able to colonize and established as endophytic in chili plant.Beauveria bassiana of wheat isolate was the best isolate giving spurring germination and seedling plant growth and not significant  with B.bassiana isolate from Leptocorisa acuta insect. Aplication of B.bassiana isolates was able to increase the chlorophyll contentof chilileaf compared control. All of the tested B.bassiana fungus could increased chlorophyl-b content and chlorophyl totally  of chili leaf, whereas chlorophyl-a content all of tested isolates was not significant. From its research  showed there were corelation between colonization and leaf chlorophyll content, the higher percentage of endophytic fungus on leaf chili,  it made higher the chlorophyl-b content and chlorophyl totally content  of leaf chili.