Edwardsiella tarda is a type of gram-negative intracellular pathogen that can cause mass demise in fish farming. The development of edwarsiellosis due to E.tarda infection causes significant economic losses for cultivators. Aloe vera is a perennial succulent plant like a cactus, drought withstand, and belongs to the Liliaceae family, of which there are more than 360 known species (Wijaya and Masfufatun, 2022). Aloe vera is one of the plants known to contain active ingredients that can inhibit the growth of bacteria. The results of phytochemical analysis of a crude extract of Aloe vera showed the presence of active compounds, specifically flavonoids, tannins, triterpenoids, and saponins. MIC analysis showed that the lowest absorbance value of Aloe vera was obtained at a concentration of 50 mg/L with a value of 0.316, indicating a decrease at each concentration. The concentration of 50 mg/L is close to the positive control which indicates that at a concentration of 50 mg/L, it can be the reference for the lowest concentration in determining the dose of Aloe vera extract which is known that the presence of antibacterial compounds in Aloe vera extract can inhibit the growth of E.tarda bacteria. The inhibition zone test was carried out with graded concentrations of 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L and 250 mg/L with an observation time of 24 hours post-incubation and 48 hours post-incubation. Inhibition zone analysis with the highest diameter was at 200 mg/L. SEM analysis by distributing a crude extract of Aloe vera affected E.tarda bacteria specifically, lysis occurring in bacterial cells
Edwardsiella tarda is a type of gram-negative intracellular pathogen that can cause mass mortality in fish farming. The development of edwarsiellosis due to E. tarda infection causes significant economic losses for fish farmers. Aloe vera as one of the plants known to contain active ingredients that can inhibit the growth of bacteria. Aloe vera has a broad spectrum of antibacterial activity, both for inhibiting gram-positive and negative bacteria (Heng et al., 2018). The results of the toxicity test of aloe vera extract showed that when used in high doses it would cause high toxic effects, namely at a dose of 200 mg/L with mortality reaching 60% of the total tested fish. The pathogenicity of E.tarda with LD50 showed that the higher the density of bacteria, the higher the possibility that E.tarda bacteria could cause disease in its host, namely at a density of 107 it experienced 50% death. The ability of E.tarda bacteria to cause disease was also influenced by its host's ability to fight pathogens. Based on the hispatological tests performed, there were changes in the morphology and behavior of the fish. Administration of aloe vera crude extract had an effect, namely being able to reduce the value of histopathological damage to the gills of gourami that had been infected with E.tarda bacteria with the lowest score, namely at a dose of 150 mg/L.
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