Amorphophallus (Araceae) contains more than 170 species that are mainly distributed in Asia and Africa. Because the bulbs of Amorphophallus are rich in glucomannan, they have been widely used in food, medicine, the chemical industry and so on. To better understand the evolutionary relationships and mutation patterns in the chloroplast genome of Amorphophallus, the complete chloroplast genomes of four species were sequenced. The chloroplast genome sequences of A. albus, A. bulbifer, A. konjac and A. muelleri ranged from 162,853 bp to 167,424 bp. The A. albus chloroplast (cp) genome contains 113 genes, including 79 protein-coding genes, 30 tRNA genes and 4 rRNA genes. The A. bulbifer cp genome contains 111 genes, including 78 protein-coding genes, 29 tRNA genes and 4 rRNA genes. A. muelleri contains 111 and 113 genes, comprising 78 and 80 protein-coding genes, respectively, 29 tRNA genes and 4 rRNA genes. The IR (inverted repeat) region/LSC (long single copy) region and IR/SSC (short single copy) region borders of the four Amorphophallus cp genomes were compared. In addition to some genes being deleted, variations in the copy numbers and intron numbers existed in some genes in the four cp genomes. One hundred thirty-four to 164 SSRs (simple sequence repeats) were detected in the four cp genomes. In addition, the highest mononucleotide SSRs were composed of A and T repeat units, and the majority of dinucleotides were composed of AT and TA. SNPs (single nucleotide polymorphisms) and indels (insertion-deletions) were calculated from coding genes and noncoding genes, respectively. These divergences comprising SSRs, SNPs and indel markers will be useful in testing the maternal inheritance of the chloroplast genome, identifying species differentiation and even in breeding programs. Furthermore, the regression of ndhK was detected from four Amorphophallus cp genomes in our study. Complete cp genome sequences of four Amorphophallus species and other plants were used to perform phylogenetic analyses. The results showed that Amorphophallus was clustered in Araceae, and Amorphophallus was divided into two clades; A. albus and A. konjac were clustered in one clade, and A. bulbifer and A. muelleri were clustered in another clade. Phylogenetic analysis among the Amorphophallus genus was conducted based on matK and rbcL. The phylogenetic trees showed that the relationships among the Amorphophallus species were consistent with their geographical locations. The complete chloroplast genome sequence information for the four Amorphophallus species will be helpful for elucidating Amorphophallus phylogenetic relationships.
The aims of this research were to study the leaf anatomy of Musa brachycarpa, M. Paradisiacal normalis, M. sapientum and M. cavendishi as well as the length, width, and thickness of the leaf and the number, diameter, and tensile strength of leaf fibers. Samples were collected in Dampit, Wajak and Batu, Malang. Indonesia. The criteria for leaf samples were that they were fresh, mature, and not torn. Microscope slides used for anatomical observations were prepared using a semi-permanent method. The Retting method was applied to extract the fibers, and fiber strength was measured using a tensile strength tester. One way Anova and the Duncan test were used to establish the mean and other parameters of the dependent variables (length-, width-, thick-leaf; number-, diameter-, and tensile strength of fiber). The T-test (independent sample) was used to determine the mean diameter of fiber in adaxial and abaxial sites. The results showed that M. Brachycarpa had the highest number of fiber cells, a wider diameter fiber, and more adaxial fiber cells than the abaxial site. The diameter of fibers was 5-6 μm. M. sapietum had the longest and widest leaves and leaf thickness was highest in M. Paradisiaca. The tensile strength values ranged from 35 × 10-4-48 × 10-4 MPa. The tensile strength of the observed species did not differ significantly.
This research had aimed to determine variations in form of calcium oxalate (CaOx) crystals in porang. Slides for microscopic observation were prepared from sliced leaf, petiole, and tuber of porang. Sliced organs were cleared by using modified clearing method. The parameters that were observed included form and variety of morphology type of CaOx crystals. Microscopic observation showed there were four basic forms of crystals, i.e., druse, raphide, prism and styloid with some variation of diverse types, each of which had 49, 10, 8, and 5 variations. CaOx crystals, based on the size, were grouped into large (20 -250 µm) and small (1 -15 µm) crystals.
Proline is one of amino acid that usually accumulates inside the plant cell when facing drought stress. The accumulation of proline can protect the plant cell from damage during drought. The aim of this research was to determine proline content and yield components of local corn cultivars from Kisar Island, Maluku, Indonesia. The field trial was organized using randomized block design with three replicates. Six local corn cultivars found in Kisar Island (Deep Yellow, Early Maturing Yellow, Red Blood, Rubby Brown Cob, Waxy, and White) were used as plant materials and a recommended tolerance variety (<em>Srikandi</em>) was taken as reference group. Proline content was determined using ninhydrin method. Yield components variables included cob weight (at harvest, after air dry, after oven dried, at 12% of water content), cob water content at harvest, cob length, cob diameter, number of seed row per cob, number of seed per cob, and cob yield at 12% of water content. Data collected was analysed with analysis of variance followed by Duncan multiple range test at the significant level 0.05 using Statistical Analysis System/SAS software version 9.0. The result shows that highest proline content and yield components (except for cob water content) was showed by the Deep Yellow cultivar. The lowest proline content was showed by Rubby Brown Cob cultivar. The lowest corn yield components was showed by Red Blood local cultivar. Deep Yellow cultivar can be proposed as superior drought tolerance variety, and can be recommended for further wide cultivation in Maluku province.
Harvest time is assumed to potentially influence shape and size variation of calcium oxalate (CaOx) crystals; therefore it needs to be observed microscopically. This research used porang corms from the second growing period which were planted to produce the vegetative phase of third growing period. These corms were obtained based on the harvest time had determined. The harvest time was determined, i.e. 1) at two weeks before the plants shed (R0 − 1); 2) when the plants shed (R0) and 3) at two weeks after the plants shed (R0 + 1). Slides for microscopic observation were obtained from slices on the edge and center of porang corms. Organ slices were cleared using modified clearing method. Parameters observed were the shape and the size of CaOx crystal. The variations of styloid, prism, druse and raphide crystals found in porang corms at the three harvest time were 1, 2, 3 and 37 variations respectively. The variation of CaOx crystals tended to be same in porang corms at three harvest times. The variation of these crystals tended to be static. It is also known that raphide crystal has the greatest variation amount. On the contrary, styloid crystal has the fewest variation amounts. This abundance of a number of raphide crystal variations is possibly due to its role as a defense mechanism in porang corms.
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