Vancomycin-resistant enterococci (VRE) have become a global problem of hospital-associated infections. A rapid VRE detection and early isolation of the positive cases are essential for prevention and control of their transmission in hospitals. The aim of this study was to develop and evaluate a simple and rapid method for detection of VRE by using 3 (4, 5 dimethyl-2-thiazolyl)-2, 5 diphenyl tetrazolium bromide (MTT) colorimetric method compared with standard broth microdilution (BMD) and Epsilometer test (Etest). A total of 85 Enterococcus isolates (75 vancomycinnon-susceptible, 2 vancomycin-intermediate and 8 vancomycin-susceptible) were studied. The minimum inhibitory concentrations (MICs) of vancomycin for all isolates were tested by BMD, MTT and Etest methods. The MTT method gave results within 9 h with good category agreement, essential agreement, very major, major, and minor errors (100, 92.9, 0, 0 and 7.1%, respectively), which were comparable to those of the Etest (97.6, 95.3, 2.4, 0 and 4.7%, respectively). The Cohen's kappa coefficient (κ) of the MTT method compared with the BMD showed perfect agreement (1.000) (p < 0.001). The MTT method is simple and rapid for early VRE detection to support prevention and control the spread of the enterococci in hospitals.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.