The study aimed to analyze the microbiological characteristics of water from artisanal wells in the municipality of Guajará in Amazonas, verifying the presence of Escherichia coli and total coliforms. The collections were carried out in residences in the center of the city in 10 points. COLItest Kits were used, which have substances, nutrients and MUG in their formulation that, properly balanced, inhibit the growth of Gram-positive bacteria, favoring the growth of bacteria from the coliform group and facilitating the identification of E. coli through fluorescence after incubation at 37 °C in 48 hours. In the qualitative analysis of the water, it was observed the presence of total coliforms in 80% of them and in 20% they presented absence of microorganisms. The samples that showed total coliforms, it was possible to observe that in 75% there was contamination by E. coli, representing a great risk to the health of those who consume water from these points without adequate treatment. However, measures for the preservation of water sources, the treatment of water for human consumption and the practice of using chlorine or boiling the water in the places where they were collected are necessary actions to reduce the occurrence of total coliforms and Escherichia coli.
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