Purpose
Necrotizing enterocolitis (NEC) is one of the most serious complications of prematurity. Many risk factors can contribute to the development of NEC. The epidermal growth factor (EGF) plays a major role in intestinal barrier function, increases intestinal enzyme activity, and improves nutrient transport. The aim of this study was to assess the role of epidermal growth factor in the development of NEC in preterm neonates.
Methods
In this study, 130 preterm neonates were included and divided into 3 groups, as follows: group 1, 40 preterm neonates with NEC; group 2, 50 preterm neonates with sepsis; and group 3, 40 healthy preterm neonates as controls. The NEC group was then subdivided into medical and surgical NEC subgroups. The serum EGF level was measured using enzyme-linked immunosorbent assay.
Results
Serum EGF levels (pg/dL) were significantly lower in the NEC group (median [interquartile range, IQR], 9.6 [2–14]) than in the sepsis (10.1 [8–14]) and control groups (11.2 [8–14],
P
<0.001), with no significant difference between the sepsis and control groups, and were positively correlated with gestational age (
r
=0.7,
P
<0.001). A binary logistic regression test revealed that low EGF levels and gestational ages could significantly predict the development of NEC. The receiver-operating characteristic curve for EGF showed an optimal cutoff value of 8 pg/mL, with 73.3% sensitivity, 98% specificity, and an area under the curve of 0.92.
Conclusion
The patients with NEC in this study had significantly lower serum EGF levels (
P
<0.001), which indicated that EGF could be a reliable marker of NEC in preterm neonates.
Extended-spectrum β-lactamase (ESBL) producing E. coli and salmonellae have spread rapidly worldwide and pose a serious threat to human and animal health. The present study was conduct-ed to determine the prevalence of ESBL-producing E.coli and salmonellae, to perform molecular characterisation of the ESBL-related bla genes, including blaTEM, blaSHV and blaCTX, and the sus-ceptibilities of these bacteria to various antimicrobial agents. From a total of 300 poultry samples, 25 and 20 samples were recognised as Salmonella and E. coli, respectively by microbiological and molecular methods. All E. coli and Salmonella isolates were positive for an ESBL phenotype. Mo-lecular detection for antibiotic resistance gene revealed blaTEM in all isolates of salmonellae and E. coli (100%), while blaSHV was detected in 5 (20%) and 2 (10%) of salmonellae and E. coli isolates, respectively. None of the isolates contained blaCTX gene. Serotyping of Salmonella spp. in chick-ens revealed that S. enteritidis was the major isolates followed by S. Infantis (21.4%), S. Kentucky (14.2%) and S. Typhimurium, S. Kapemba, S. Newport, S. Vejle and S. Magherafelt were detected at 7.1% respectively. S. Infantis was the major isolate detected in chicks (60%), while in ducks S. Typhimurium and S. Blegdam were identified. In ducklings, S. Sinchew, S. Infantis and S. Sekon-di were equally prevalent. Only S. Newmexico was identified in poultry products. E. coli in chick-en were serotyped into O1, O8, O29, O125, O128 and O157. In chicks, O29 and O126 serotypes were detected. In poultry products only O8 was detected. The results indicate that ESBL frequen-cy has reached an alarming level in poultry isolates in Egypt, with TEM enzymes being the pre-dominant β-lactamases detected.
Background
Although some investigators have confirmed the association between H. pylori and chronic ITP in adults, studies in pediatric patients are still few and have produced conflicting results. The study was carried out to detect the prevalence of H. pylori among chronic ITP children and to investigate the impact of treatment of H. pylori infection on platelet count response.
Results
The prevalence of H. pylori in chronic ITP children was 63%. The platelet count was statistically significantly higher among H. pylori stool antigen (HpSA)-negative children. A significant difference was reported in which platelet count increased from 70.55 ± 4.788 million/μL before H. pylori eradication therapy to 110.78 ± 15.128 million/μL after therapy.
Conclusion
We concluded that H. pylori eradication therapy was effective in increasing platelet count in H. pylori-positive chronic ITP patients.
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