Divalent DNA-AuNP (gold nanoparticle) conjugates comprising two DNA strands at diametrically opposed positions are prepared. Highly linear 1D and tetragonal lattice-like 2D AuNP arrays are constructed using the conjugates and DNA assemblies based on T- and double-crossover motifs and the Holliday junction.
Micro- and nano-scale fabrications of various fluorinated polymers were demonstrated by direct maskless etching using a focused ion beam (FIB). The etching rates of perfluorinated polymers, such as poly(tetrafluoroethylene) (PTFE), poly(tetrafluoroethylene-co-hexafluoropropylene) (FEP), poly(tetrafluoroethylene-co-perfluoroalkoxyvinylether) (PFA), were about 500–1000 times higher than those of partially fluorinated polymers, such as poly(tetrafluoroethylene-co-ethylene) (ETFE) and poly(vinilydene–fluoride) (PVdF). Controlled high quality and high aspect-ratio nanostructures of spin-coated cross-linked PTFE were obtained without solid debris. The height and diameter of the fibers were about 1.5 µm and 90 nm, respectively. Their aspect ratio was about 17.
A simple and conventional method to isolate gold nanoparticle (GNP)/oligo-DNA conjugates having defined number of oligo-DNAs by gel electrophoresis was developed using extension oligo-DNAs. Formation of self-organized assemblies of GNPs in two different sizes using the conjugates having one or two complementary oligo-DNAs was demonstrated. This method to prepare GNP/oligo-DNA conjugates with controlled number of DNA strands should be useful to build up functional nanoarrays of GNPs on DNA scaffolds.
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