We herein report the first study into the use of an enzyme mixture of cellulase and β-glucosidase for cellulose hydrolysis, with the overall aim of expressing the effect of β-glucosidase addition using a simple equation. To achieve this goal, different amounts of commercial cellulase and β-glucosidase were added to a cellulose slurry, and hydrolysis experiments were carried out at 40 and 50 over 0-48 h in a shaker incubator using a 0.1 mol/ dm 3 sodium acetate solution to maintain a solution pH of 5. The glucose yield got higher for the larger amount of cellulase and larger amount of β-glucosidase. The yield of glucose recovered at 50 was lower than that recovered at 40 . Finally, an equation to estimate glucose production using such a cellulase and β-glucosidase mixture was then developed for the first time, and this equation correlated well with the experimental data. The obtained equation expressed the hydrolysis using another bottle of cellulase, too.
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