Our results suggest that GSDMC is increased by UV radiation and contributes to UV-induced MMP-1 expression through the activation of ERK and JNK pathways.

Gasdermin (GSDM)‑C is a member of the GSDM gene family and is expressed in the epithelial cells of various tissue types, including skin. GSDMC expression is induced by ultraviolet (UV) irradiation and contributes to UV‑induced matrix metalloproteinase 1 expression in human skin keratinocytes. However, how UV irradiation induces GSDMC expression remains unclear. The present study aimed to investigate the role of transient receptor potential cation channel subfamily V member 1 (TRPV1) and a calcium/calcineurin‑signaling pathway in UV‑induced GSDMC expression in human skin keratinocytes. Suppression of TRPV1 activity by treatment with the TRPV1 antagonists capsazepine and ruthenium red significantly reduced UV‑induced GSDMC expression, whereas direct activation of TRPV1 by capsaicin, a TRPV1 agonist, increased GSDMC expression. The results demonstrated that extracellular calcium and calcineurin activity may be necessary for UV‑induced GSDMC expression in HaCaT cells. In addition, UV‑induced GSDMC expression was either decreased or increased following knockdown or overexpression of nuclear factor of activated T‑cells, cytoplasmic 1 (NFATc1), respectively. These data suggested that TRPV1 may serve an important role in the induction of GSDMC expression by UV and that UV‑induced GSDMC expression may be mediated via a calcium/calcineurin/NFATc1 pathway.

Keloids, benign cutaneous overgrowths of dermal fibroblasts, are caused by pathologic scarring of wounds during healing. Current surgical and therapeutic modalities are unsatisfactory. Although adiponectin has shown an antifibrotic effect, its large size and insolubility limit its potential use in keloid treatment. We investigated the effect of a smaller and more stable adiponectin-based peptide (ADP355) on transforming growth factor β1 (TGF-β1)-induced fibrosis in a primary culture of keloid fibroblasts prepared from clinically obtained keloid samples. Xenograft of keloid tissues on athymic nude mice was used to investigate the effect of intralesional injection of ADP355. ADP355 significantly attenuated the TGF-β1-induced expression of procollagen type 1 in keloid fibroblasts (p < 0.05). Moreover, it inhibited the TGF-β1-induced phosphorylation of SMAD3 and ERK, while amplifying the phosphorylation of AMP-activated protein kinase (p < 0.05). Knockdown of adiponectin receptor 1 reversed the attenuation of procollagen expression in ADP355-treated TGF-β1-induced fibrosis (p < 0.05). ADP355 also significantly reduced the gross weight and procollagen expression of keloid tissues in xenograft mice compared to control animals. These results demonstrate the therapeutic potential of the adiponectin peptide ADP355 for keloids.

Background: DotA-2 is the most popular MOBA video game, which ranks the second most sold on the steam platform. Reaction time as a physiological parameter to find out how quickly to respond to a stimulus. Some research states that video games can increase reaction time. Objective: To know the correlation of playing video game Defense of the Ancients-2 with the duration of reaction time. Method: The study used cross-sectional design. This study was conducted in the area of the Faculty of Medicine, Diponegoro University. In this study found 42 respondents participated. Score of reaction time is measured using a ruler dropped from the respondent's fingertips. Analysis using  Shapiro-Wilk test and Pearson test. Results: There is significant relationship between playing a DotA-2 status and reaction time. The duration of playing DotA-2 video games to the reaction time there were significant differences between the 3 groups. Conclusion: There is a significant relationship between the status of playing video game DotA-2 with reaction time.

Background: Acne vulgaris (AV) is an inflammatory condition of pilosebaceous unit that is common in adolescents and young adults. The pathogenesis of AV is multifactorial, one of which is inflammation. Interleukin-23 (IL-23) has an important role in the activity of T helper 17 (Th17) cells that produce IL-17 in chronic inflammation.Objective: To determine the correlation between serum IL-23 levels and AV severity in men.Methods: This is an analytical observational preliminary study with a cross-sectional design involving 15 male subjects with AV who were divided into groups of AV severity based on Lehmann’s criteria. The subjects were examined for serum IL-23 levels. Data were analyzed using Spearman’s Rho non-parametric correlation test to determine the correlation between variables.Results: Mean IL-23 serum levels in men with AV was 111,05 ± 115,27 pg/ml, with mild AV was 210,57 ± 122,038 pg/ml, moderate AV was 103,65 ± 91,15 pg/ml and severe AV was 18,94 ± 15,76 pg/ml. There is a significant correlation between IL-23 serum levels and AV severity in men. (p = 0,001). Serum IL-23 levels decreased with increasing AV severity (r = -0,756).Conclusion: There is a strong negative correlation between IL-23 serum levels and AV severity in men.