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Nitrogen is the most abundant element in the atmosphere, however, most often deficient in agricultural lands. This research was an exploratory to get indigenous non-symbiotic nitrogen-fixing bacteria. Soil samples were collected from rhizosphere of green beans. This study was aimed to determine the bacterial population of the three regions; screening, isolation and selection of free nitrogen-fixing bacteria. Antagonism and pathogenicity tests were performed to observe its potential for a biofertilizer product. The highest number of free nitrogen-fixing bacteria was found from forest soil sample of 2.5x 10 11 CFU/ml. Screening and isolation process has obtained 10 free nitrogen-fixing isolates. Then was selected into 4 isolates namely SNF4, SNF5, SNF7 and SNF8 according to the ammonia production test qualitatively. When an antagonism activity performed, there was no inhibition zone each other. The pathogenicity test did not show the pathogenic symptom. This study also showed that bacterial isolates obtained significantly affected the germination growth of green beans compared to controls. Possibility, bacteria of this type produced growth hormone for a plant. Strain SNF8 has shown the highest ammonium production then was selected for 16S rRNA identification. Similarity test of genome sequence of strain SNF8 had 99% similarity with Bacillus cereus.

:The use of phosphate-solubilizing bacteria (PSB) as a biological fertilizer of Agricultural land is one solution to overcome problem of phosphate availability for plants. However, often application of a biological fertilizer is ineffective for certain places. The purpose of this study was to obtain indigenous phosphate solubilizing bacterial isolates that can be effective in the area of Malang. Samples were collected from rhizosphere of green bean plants at three locations in Malang, East Java. The study was conducted to determine the total bacterial population of soil samples, to select the best three bacterial isolates in phosphate solubilizing ability, which is not antagonistic and nonpathogenic for plants, along with observing its potential as a bacterial consortium. The highest total population was found in FHR samples of 1.5x10 11 CFU / mL. We have selected three bacterial isolates namely SPP1, SPP2 and SPP3. They were not antagonistic to each other and nonpathogenic on mungbean sprouts. They had possibility of producing growth hormone which characterized by an increasing in length of plant and total root length, be compared to controls. Strain SPP2 has shown the highest activity of phosphate solubilization then was selected for 16S rRNA identification. Similarity test of genome sequence of strain SPP2 had 99% similarity with Pseudomonas plecoglossicida strain PR19.

In this current study, we observed Trichoderma viride strain FRP3 capability for biodegradation of glyphosate on contaminated land in Indonesia. There were two blank plots that have been involved as representatives of indigenous fungal, that prepared as control (non-contaminated soil) and P1 (GP-contaminated soil) while the treatments were represented by two plots. Plot 2 (P2) was introduced with conidia suspension of Trichoderma viride strain FRP3 one time application, and plot 3 (P3) was introduced with conidia suspension of Trichoderma viride FRP3 two time applications. At the end of observation, the CFU of two times application was the highest with CFU of 15.97 x 10 6 gr -1 soil. The CFU of P3 was corresponding to 45% higher than P2 (8.83 x 10 6 gr -1 soil). The CFU of GP-contaminated soil without conidia suspension application had 0.66 x 10 6 gr -1 soils, only 0.7% and 0.4% corresponding to P2 and P3, respectively. Direct indicator of glyphosate degradation was determined using GC analysis. Within 7 days after Trichoderma viride FRP3 was introduced, glyphosate content of treated soil decreased. This fungal strain provided 48% (P2) and 70% (P3) of glyphosate degradation higher than indigenous soil microbial community (P1) within 28 days of application.

:Steady soil aggregationis important for agricultural land which is formed by the micro-

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Glyphosate herbicide was used as screening agent to be the sole source of phosphorus for the isolation of soil-born fungi of forest soil. Three fungal strains were able to grow consistently in the presence of glyphosate as the sole phosphorus source and were identified as Fusarium sp. strain FRP1, Scopulariopsis sp. strain FRP2 and Trichoderma sp. strain FRP3. On standard medium ten fungal strains were isolated and identified as Botrytis sp. strain FR1, Mucor sp. strain FR2, Acremonium sp. strain FR3, Trichoderma sp. strain FR4, Botrytis sp. strain FR5, Crysosporium sp. strain FR6, Scopulariopsis sp. strain FR7, Trichoderma sp. strain FR8, Botrytis sp. strain FR9 and Acremonium sp. strain FR10. Of the three screened fungal species, Scopulariopsis sp. strain FRP2 and Trichoderma sp. strain FRP3 were selected for further study because they had the highest ratio of growth diameter. The growth kinetics of Scopulariopsis sp. strain FRP2 and Trichoderma sp. strain FRP3 were also observed on broth Czapek medium containing glyphosate as the sole source of phosphorus. Synergetic interactions in vitro were examined on PDA. The synergetic interactions of Scopulariopsis sp. strain FRP2 and Trichoderma sp. strain FRP3 as mix culture showed antibiosis inhibition mode.