Research on the extraction and stability test of natural dye from red spinach (Alternanthera amoena Voss) has been carried out. This study aims to determine the effect of pH and length of sun exposure on the stability of the dye from red spinach. Extraction using the maceration method with ethanol solvent and measurement using a UV-Vis spectrophotometer. Stability testing is carried out at pH 2-5 and sun exposure for 1-4 hours using a dark and light container. The results obtained, at pH 2 and pH 3, anthocyanins were more stable, compared to pH 4 and 5 which had greater color degradation. In exposure to sunlight, dark containers with a degradation percentage of 31.70% were better than clear containers with a percentage of 48.78%.
Keywords: Anthocyanin, red spinach, natural dyes stability.
This research was conducted by making VCO-based liquid soap with the addition of ethanol extract from Moringa leaves (Moringa oleifera). The purpose of this research was to determine the mass ratio of ethanol extracts of Moringa leaves and Virgin Coconut Oil (VCO) which produces soap with the highest antibacterial activity against tested bacteria. The other purpose is to determine the effect of the mass ratio against the viscosity of liquid soap. The research design used was a Completely Randomized Design (CRD) with the independent variables being the mass ratio of Moringa leaf ethanol extract and VCO which consists of five levels of 0:75 (F1), 1.5:73.5 (F2), 3:72(F3), and 4.5:70.5(F4) (w/w). The soap produced was tested for antibacterial activity againts Staphylococcus aureus, Escherichia coli, and Staphylococcus epidermidis using agar diffusion method as well as viscosity tests. The results showed that the antibacterial activity of liquid soap tends to be higher with increasing levels of ethanol extract of Moringa leaves. The liquid soap formula with the ratio of Moringa leaf ethanol extract: VCO of 3:72 (w/w) (Formula F3) had relatively higher antibacterial activity against the three tested bacteria. The inhibition zones against Staphylococcus aureus, Escherichia coli, and Staphylococcus epidermidis were 22.302 mm, 33.230 mm, and 36.490 mm, respectively, while the viscosity value of liquid soap was 47.433 cP.
Research on the antibacterial activity of ethanol extract from seedcoat of local Sorghum (Sorghum bicolor [L.] Moench) against Staphylococcus aureus and Escherichia coli has been conducted. The purpose of this study was to determine the minimum inhibitory concentration of extract against both gram-positive and negative bacteria. The extraction method used in this study was a maceration method using ethanol as a solvent. Antibacterial activity test was carried out with several concentrations, namely 1 g/mL, 0.8 g/mL, 0.6 g/mL, 0.4 g/mL, 0.2 g/mL, 0.1 g/mL, 0.05 g/mL, 0.025 g/mL, and 0.01 g/mL. The results showed that the extract inhibited bacterial growth in a wide range of concentrations. The smallest inhibition was at a concentration of 0.05 g/mL with a clear zone diameter of 10.29 mm in Staphylococcus aureus and 11.70 mm in Escherichia coli. Ethanol extract of local Sorghum seedcoat exhibited a strong inhibition ability and probably a potential antibacterial agent source.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.