Background: Nurses play a crucial role at poisoned center unites, as they are responsible for administering nursing care to poisoned children. Aim of the study was to identify effect of guideline protocol for nurses' performance regarding care provided toward poisoned pre-school age children. Design: A quesi experimental research design was utilized in the current study. Setting: The current study was conducted at Benha poisoning center in University Hospital at Benha city. Sample: All nurses work in poison center 50 nurses was participated in the current study. Tools of data collection (I): A structured interviewing questionnaire consisted of three parts, part (1): personal data for nurses, part (2): personal data for pre-school age and part (3): nurses' knowledge regarding poisoned children and Tool (II): An observational checklist of selected nursing procedures to evaluate the practice of the nurses regarding poisoned children. Results: More than two third of studied nurses had poor level of total knowledge scores and the most of them had incompetent in their total performance scores also, there was a positive correlation between total knowledge and practice score regarding nursing care procedure in pre and post guideline. There was no statically significant relation between of the studied nurses' total knowledge scores and their personal characteristics, there was no statically significant relation between of the studied nurses' total performance scores and their personal characteristics. Conclusion:There was a highly statically significant improvement in the studied nurses' knowledge and practice score regarding poisoned preschool age guideline protocol intervention when compared with pre guideline protocol intervention. Recommendations: Adding poisoning course nursing curriculum.
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