Vitiligo is a rare skin condition caused by an immune reaction. Vitiligo can occur anywhere on the body. This proposed explanation of vitiligo makes it clear that vitiligo is not linked to any other autoimmune diseases. The polymorphisms of some genes present in the immune system play a major function in susceptibility of vitiligo. Meta-analysis studies have shown that the Angiotensin converting enzyme (ACE) gene insertion and deletion polymorphism is closely associated with vitiligo in many ethnicities. The connection between ACE gene and vitiligo is connected through the auto immune diseases and there are no genetic polymorphism studies have been carried out with ACE gene with vitiligo in the Saudi population. Previous studies show that vitiligo patients are more likely to also have an autoimmune disorder. The current study aims to investigate the I/D polymorphism in the ACE gene with diagnosed patients with vitiligo subjects. This is a case-control study carried out in the Saudi population with 100 vitiligo cases and 100 healthy controls. Genotyping was performed through polymerase chain reaction followed by 3% agarose gel electrophoresis. Genotype and allele frequencies were carried out with genetic mode of inheritances. Statistical analysis was performed considering p < 0.05 as significant association. There was a substantial difference in allele frequency distribution between vitiligo patients and healthy controls (OR-1.70 (95%CI: 1.14–2.53); p = 0.008). Additionally, DD genotype (OR-4.71 (95%CI: 1.42–15.61); p = 0.008) and recessive model (OR-2.66 (95%CI: 1.41–5.02); p = 0.002) was strongly associated. Both dominant and co-dominant showed the negative association (p > 0.05) when compared between the vitiligo cases and controls. The correlation between age and genotyping was performed with Anova analysis and current study results confirmed the substantial link between 11 and 20 years (p = 0.01) and 31–40 years (p = 0.04) with the defined age groups. In conclusion, in Saudi populations, the ACE gene I/D polymorphism was identified as being correlated with vitiligo. This is the first study in Saudi Arabia to report the risk factors of vitiligo with the ACE gene polymorphism.

The most severe clinical manifestations of the horrifying COVID-19 disease, that claimed millions of lives during the pandemic time, were Acute respiratory distress syndrome (ARDS), Coagulopathies, septic shock leading eventually to death. ARDS was a consequence of Cytokine storm. The viral SARS-COV2infection lead to avalanche of cytokines and eicosanoids causing “cytokine storm” and “eicosanoid storm.” Cytokine storm is one of the macrophage-derived inflammatory responses triggered by binding of virus particles to ACE2 receptors of alveolar macrophages, arise mainly due to over production of various pro-inflammatory mediators like cytokines, e.g., interleukin (IL)-1, IL-2, and tumor necrosis factor (TNF)- α, causing pulmonary edema, acute respiratory distress, and multi-organ failure. Cytokine storm was regarded as the predictor of severity of the disease and was deemed one of the causes of the high mortality rates due to the COVID-19. The basis of cytokine storm is imbalanced switching between an inflammation increasing - pro-inflammatory (M1) and an inflammation regulating-anti-inflammatory (M2) forms of alveolar macrophages which further deteriorates if opportunistic secondary bacterial infections prevail in the lungs. Lack of sufficient knowledge regarding the virus and its influence on co-morbidities, clinical treatment of the diseases included exorbitant use of antibiotics to mitigate secondary bacterial infections, which led to the unwarranted development of multidrug resistance (MDR) among the population across the globe. Antimicrobial resistance (AMR) needs to be addressed from various perspectives as it may deprive future generations of the basic health immunity. Specialized pro-resolving mediators (SPMs) are generated from the stereoselective enzymatic conversions of essential fatty acids that serve as immune resolvents in controlling acute inflammatory responses. SPMs facilitate the clearance of injured tissue and cell debris, the removal of pathogens, and augment the concentration of anti-inflammatory lipid mediators. The SPMs, e.g., lipoxins, protectins, and resolvins have been implicated in exerting inhibitory influence on with cytokine storm. Experimental evidence suggests that SPMS lower antibiotic requirement. Therefore, in this review potential roles of SPMs in enhancing macrophage polarization, triggering immunological functions, hastening inflammation resolution, subsiding cytokine storm and decreasing antibiotic requirement that can reduce AMR load are discussed.

Mosquito control remains a powerful method to control mosquito-transmitted diseases. Focusing on early stages is very important for many reasons because they are easy to handle, significantly affected by ambient environmental influences, natura.032.0l, and chemically toxic. The current study was aimed to examine the chemical components and larvicidal effect of Ethanolic extract of O. basilicum leaves and flowers on mosquito larvae. Fresh Ocimum basilicum was collected, dried in the shade for one week, and extracted with ethanol. The larvae were placed in plastic containers and bioassayed according to the protocol approved by WHO. After 24 hours of the recovery period, mortality percentage was recorded. Dead larvae were examined under a microscope to observe any morphological changes. The statistical analysis using SPSS program version 19 was done to determine Probit data. The phytochemical investigation of O. basilicum leaves presented many components such as flavonoids, glycosides, tannins, and steroids, whereas alkaloids, saponins, and terpenoids were absent. Moreover, O. basilicum flowers showed the presence of only tannins, terpenoids, steroids, and flavonoids, but not Saponins, glycosides alkaloids. The action of leaves and flower extracts were given LC50 values of 17.

Aggrandize industrialization and urbanization have resulted in many issues, such as increased energy demand, a plethora of waste output, and negative environmental consequences. As a result, there is excessive exploitation and over-usage of fuels and finite resources, which is paving the path for the exhaustion of fuels. Extensive use of these fossil-derived fuels has caused serious threats to the environment in terms of greenhouse gases emission leading to breathing troubles and other associated health hazards. In order to mitigate the harmful effects of fossil-derived fuels, researchers are more focused towards the production and application of bio-based fuels like bioethanol, biodiesel, biohydrogen etc. These biofuels are produced from crops and edible/non-edible materials and emit much lower pollution compared to fossil-derived fuels. Even though biofuels are effective alternatives, high operational costs with low production volume are the major limitations of this process, which the available technologies cannot handle. With increasing application of nanoparticles as catalysts in several sectors due to its unique properties such as high catalytic activity, surface to volume ratio, mechanical properties, etc., its application in biofuels production has been explored recently. The present review focuses on the application of nanocatalysts in various stages of biofuel production, different types of nanocatalyst used in the innovative era and for biofuels production and their merits and demerits. The supply of biofuels, such as feedstock is large, and with improved processing, we may be able to significantly lower our reliance on fossil fuels. The present review discusses the current updates, future possibilities, and challenges of biofuels production to help make the country self-reliant in the field of green energy.

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