Morphological changes at the cell level have been produced experimentally by a pulsed ruby laser (optical maser). A pulse duration of approximately 500 microseconds caused discrete damage to structures in the cell without irreversible damage to the surrounding area.
SUMMARY
This paper addresses the nutrition and relative importance of primary production to heterotrophy of select unicellular green algae and diatoms from the same assemblage. Algal growth responses to nutrient additions varied widely. Such responses included: inhibition and enhancement of growth by amino acid additions: nonstimulation or inhibition by most sugars 10 mM concentration; glucose stimulation of 6 and inhibition of 3 species; fructose inhibition of 6‐ and 3‐ fold stimulation of one species; stimulation of most species by a vitamin mixture, some natural products, metabolite mixtures, etc. Photoassimilation of glucose and aculeate in 8 of 12 species occurred. Nine of 12 species took up only a fraction of the total carbon fixed as organic substrate. Enhancement of photosynthesis by glucose and inhibition by acetate was common. The data suggest that attached littoral and shoal marine algal assemblages may play mixed trophic roles lower levels of the detrital food web.
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