This report highlights a rare case of acute hydrops in a host cornea that developed 7 years after penetrating keratoplasty (PKP) for keratoconus. We obtained detailed clinical findings of the condition by anterior segment optical coherence tomography (AS-OCT). We believe this is the first report of hydrops localized in the host cornea without causing edema in the graft. In addition, its onset was the earliest observed after keratoplasty [1-4].
Case reportA 37-year-old man had been followed up by a local doctor for keratoconus since 1995. The patient developed acute hydrops in his right eye in 2001 and was referred to our hospital by the local doctor. The acute hydrops improved spontaneously after 2 months. His best corrected visual acuity (BCVA) oculus dexter (OD) with a hard contact lens (HCL) recovered to 1.2. Later, the patient developed acute hydrops OD twice, and his BCVA decreased to 0.2. He underwent PKP in his right eye at our hospital in 2003. In 2005, he underwent PKP in his left eye because of HCL intolerance due to an exacerbation of the keratoconus. In 2009, his BCVA with HCL was 1.5 oculus uterque (OU). The graft and host cornea OU were clear. The corneal endothelial cell density (ECD) was 573 cells/mm 2 OD and 871 cells/mm 2 oculus sinister (OS); the central corneal thickness (CCT) was 610 lm OD and 590 lm OS. The patient revisited our hospital in January 2010 complaining of corneal opacity in the nasal side of the right eye and HCL intolerance. Despite decentration of the HCL to the nasal side, VA OD was 1.2. Although slit-lamp microscopy revealed corneal opacity and thickening of the lower nasal side of the host cornea, the graft was transparent, and no inflammatory or rejection findings were observed (Fig.
By using a commercially available surface plasmon resonance (SPR) biosensor, the values of the association rate constant (kass), dissociation rate constant (kdiss), and association constant (KA = kass / kdiss) for binding to the antigens were determined. The were almost the same for the recombinant antibody expressed in COS cells, CH 6 cells, and mouse hybridoma cells. The system of transient expression of the recombinant antibody (Ab) in COS ceils and SPR analysis of the supernatant should be useful for rapid expression and evaluation of the binding ability of large numbers of engineered Abs.
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