Leishmaniasis diseases are endemic in Morocco. An entomological survey was conducted in Aichoun locality for 1 year from September 2013 to August 2014. The objective of this study was to investigate the sand fly fauna, mainly the species composition and the monthly species prevalence in accordance with bioclimate factors. Sand flies were collected twice a month, using sticky traps and CDC light traps. During a one-year study, 4472 specimens of sand fly were caught (72.56% male/22.44% female) that were composed of seven species divided into two genera: Phlebotomus (99.46%) and Sergentomyia (0.53%). Ph. sergenti was the most prevalent species (46.64%), followed by Ph. perniciosus (38.19%), Ph. longicuspis (9.32%), Ph. papatasi (5.23%), and Ph. ariasi (0.06%). The genus Sergentomyia was even less frequent. The population dynamics showed a bimodal trend with two peaks: the first one in October (12.03% of specimens) and the second in June (27.92% of specimens). The study of the effects of climatic factors in the study area showed a link between the dynamics of sand flies and the variation of these parameters (temperature and relative humidity). During the period between November and March, the sand flies were absent. The highest prevalence of sand flies was recorded in June when the temperature and relative humidity values reached, respectively, 25.8°C and 42%. The minimum number of specimens was collected in September with an average temperature of 23.19°C and relative humidity of 57.4%. Statistical analysis with principal component analysis has shown a strong positive correlation between temperature parameters and the seasonal distribution of sand flies. The climatic factor of relative humidity has been judged of being negatively correlated. The wind speed does not have any impact on the relative abundance of all species. Within this context, the results will be useful for the development of a monitoring program to better manage the operations and evaluate their effectiveness.

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The chemical characterization of the Ghis-Nekor groundwater has become a concern of many researchers in Morocco. It is a crucial indicator for the environment situation and the socioeconomic development of this Moroccan region. Indeed, it helps decisionmakers carry out conscious and sustainable management. For this reason, 20 samples of the Ghis-Nekor aquifer were examined in terms of physicochemical parameters such as pH, temperature, electrical conductivity (EC), and total alkalinity (Alk), major cations–anions (Ca2+, Mg2+, Na+, K+, HCO3−, Cl−, SO42−, NO3−, and TDS), isotopic elements (δ18O, δ2H, and δ13C), and concentrations of Br and Sr anions in 2020. Furthermore, spatial data analysis with a geographic information system (GIS) using the ArcGis software (Redlands, California, USA). Indeed, static maps show significant water quality information that helps characterize the groundwater of the study area. The spatial analysis by the GIS indicates that except the EC (from 2630 to 6950 mS/cm), all the physical parameters showed standard concentration values in most of the samples. A significant concentration above the Moroccan norm of groundwater quality for sodium (Na+) from 264.3 to 1500 mg/L, chlorides (Cl−) concentrations from 408.3 to 1510 mg/L, SO42− from 313.1 to 999.2 mg/L, and bicarbonate (HCO3−) from 283.7 to 679.8 mg/L was observed, while all the points exceeded the recommended standards norm for SO42−. Most of the points met the potability standards for potassium (K+). In terms of the isotopic elements, the concentration values of δ18O were from −3.92‰ to –5.60‰, while the δ2H concentration values varied between −28.67‰ and −39.99‰. The analysis revealed values of carbon isotope δ13C ranging between −3.15‰ and −8.61‰, thus suggesting that the origin of the carbon is mineral, mainly deriving from calcite dissolution. The analysis of the bromide and strontium contents made it possible to discriminate the origin of the salinity anomalies. The Cl/Br, Br/Cl, and Sr/Ca molar ratios distinguished the areas of influence of geological setting (saliferous facies outcrops) or anthropogenic effect (wastewater). The results of the analysis shed light on factors of contamination, which are as follows: urban zones (Imzourene, Ait Youssef, and Souani) and agricultural activities. Therefore, the use of these waters could pose a risk to the health of humans and animals. Similarly, the GIS is a practical and effective tool for the Ghis-Nekor groundwater quality diagnostics and could help decisionmakers establish solutions.