Cancer can be classified as a fourth leading cause of death in Malaysia. There is a continuous effort by scientists in finding alternative cure to cancer due to the known side effects of chemotherapy and radiation therapy as well as recurrences. One of the latest methods to kill cancerous cells is by using immune cells known as natural killer (NK) cells. Flavonoids such as flavone and flavonol are also known for their antioxidant, anti-inflammatory, immunomodulatory and anticancer properties. This study was carried out to determine the role of flavonoid compounds of apigenin, luteolin, and quercetin to facilitate the growth of NK-92 cells. NK-92 cell line was grown in tissue culture flasks containing α- Minimum Essential Medium (MEM) medium enriched with L-glutamine, 12.5% fetal bovine serum, 12.5% horse serum, 0.2 mM myo-inositol, 0.02 mM folic acid, and 100 - 200 U/mL recombinant interleukin 2 (IL-2). The cell viability was determined via trypan blue staining where the cells were manually counted by a haemocytometer. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cell viability of NK-92 cells after treatment with apigenin, luteolin, and quercetin. Results showed a dose-dependent proliferative effects of apigenin, luteolin, and quercetin on the proliferation of NK-92 cells showing the highest percentage of proliferation at 100 μg/mL for all compounds (*P < 0.05). However, exceeding the dose of 100 μg/mL had resulted in a decline of cell proliferations percentage. Based on these findings, flavonoid compounds comprising apigenin, luteolin and quercetin were able to induce proliferative effects on NK-92 cells.
Background: A new approach involving immune-cell-mediated cancer therapy has been adopted extensively for the sake of lung cancer treatments by utilizing natural killer (NK) cells. NK cell activity can be enhanced with certain agents, and among them are flavonoids. Thus, this study was conducted to investigate the immunomodulatory roles of apigenin, luteolin and quercetin on NK cell activity against lung cancer cells and on the secretions of perforin and granulysin profile. Methods: The NK-92 cells were grown in complete α-Minimum Essential Medium (MEM). NCI-H460 lung cancer cells were cultured in Roswell Park Memorial Institute 1640 media. NK cell activity against lung cancer cells were done using MTT(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The secretions of perforin and granulysin profiles were then analysed using enzyme-linked immunosorbent assay. Results: Apigenin, luteolin and quercetin significantly increased the NK-cell-mediated cytotoxic activity against lung cancer cells at concentrations 12.5 µg/ml and 25µg/ml ( P < 0.001). The secretion levels of perforin and granulysin from NK cells were also significantly enhanced with apigenin and luteolin treatment but not with quercetin. Conclusions: All three flavonoid compounds possessed some significant immunomodulatory actions on NK cell cytotoxic activity and granule secretion profiles towards lung cancer therapy.
Honey has been used as a supplement nutrient in human for centuries. It exerts antibacterial, anticancer, antiinflammatory properties as well as analgesic activity. But not many studies have been done to analyze effect of honey on morphine tolerance. Hence, this review is targeting on analgesic effect of honey bioactive compounds and their potential in morphine tolerance study.
Opioid dependence is one of the severe social problems encountered by civilization nowadays. Prior to the illicit use of opioid, an abundance of research had been done in order to understand the molecular and cellular action of the opioid. In spite of that, the actual cellular mechanism remains controversial. Hence, this review is targeting on the mechanism of opioid dependence in a biochemical pathway and treatments available either via drug medication or the use of natural remedies to treat opioid dependence.
Legumes are the plant that contains high protein content that has been used as supplementary for animal feed and has a great potential to increase the productivity of livestock. However, no much study has been measured on the nutritive value of legume species. Thus, the aims of this study were to measure the nutritive content in the leaves of three different legume species (Leucaena leucocephala, Calopogonium mucunoides and Stylosanthes guianensis) and to compare the species that contain high of nutritive value. The samples were collected in Tembila area, Besut, Terengganu, then were brought to the Plant Physiology laboratory at UniSZA Kampus Besut for sample preparation. The samples were washed under tap water for 30 seconds to prevent nutrient leaching, then were dried and ground before analyzed by proximate analysis and mineral analysis using Induces Coupled Plasma Optical Emission Spectrometry (ICP-OES) machine. The parameters that were measured by using the proximate analysis consist of dry matter (DM), crude protein (CP), crude fibre (CF), ash, ether extract (EE) and Nitrogen Free Extract (NFE). For mineral analysis, the samples were analyzed for Calcium (Ca), Zinc (Zn), Iron (Fe), Copper (Cu) and Manganese (Mn). The results of this study showed that Leucaena leucocephala had significantly higher (p < 0.05) of CP and EE. While, Stylosanthes guianensis had significantly higher (p < 0.05) of the micro-elements which are Zn, Fe and Mn. Therefore, this study revealed that L. leucocephala contained the highest nutritive value of CP, EE and Calcium.
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