The bacterial isolates were obtained from Al-Kindi Hospital were diagnosed by the Vitek-2 system and re confirm by 16srRNA gene as S. aurous, the results were shown 20 isolates (66.7%) out of 30 isolates were positive to protease production. All bacterial isolates (100%) were sensitive to Gentamicin and Levofloxacin. but resistant (100%) to aztreonam. The best temperature for enzyme production from bacteria was 37 °C, and the best pH for enzyme production was 7. Partial purification of the bacterial enzyme (protease) was carried out using short steps included ammonium sulfate 65% saturation, ion exchange using DEAE- cellulose column and then applied on gel filtration chromatography using Sephadex G-200 column. The enzymatic activity was determined for each purification step. The specific fold and yield of the purified enzyme were 5.91 and 38.3 % respectively. The molecular weight of the purified enzyme was 37 kDa , it was determined by SDS-PAGE. After being exposed to high concentrations of the protease enzyme (800-1000 µg/ml), the proliferation of a breast cancer cell line (MCF-7) was seen to be suppressed, however the inhibitory effect gradually diminished as the concentration of the enzyme decreased. 200–400 µg/ml is the recommended concentration.

Aim of this study was to assess the Seroprevalence of Cytomegalovirus (CMV), Rubella, Herpes simplex II and Chlamydia trachomatis infections among pregnant and abortion women. This study was performed on 210 women who attended the gynecological consultative clinic in Salah El-Din general hospital during November 2021 until the end of march 2022.the sera samples were tested by ELISA assay for detection of specific IgG and IgM antibodies. Overall Seroprevalence was 10%, 5.7%, 3.3%, 1.9% of IgG for CMV, Rubella, Chlamydia trachomatis and HSV II respectively while IgM was 3.8%, 2.8%, and 1% for the last two respectively.