This two-year study observed the influence of various barley-based cropping systems on soil physicochemical properties, allometric traits and biomass production of barley sown under different tillage systems. Barley was cultivated in different cropping systems (CS), i.e., fallow-barley (fallow-B), maize-barley (maize-B), cotton-barley (cotton-B), mungbean-barley (mungbean-B) and sorghum-barley (sorghum-B) under zero tillage (ZT), minimum tillage (MT), strip tillage (ST), conventional tillage (CT) and bed-sowing (BS). Interaction between different CS and tillage systems (TS) positively influenced soil bulk density (BD), total porosity, available phosphorus (P), ammonical and nitrate nitrogen (NH4-N and NO3-N), available potassium (K), allometric traits and biomass production of barley. The highest soil BD along with lower total porosity were noted in ZT leading to lesser leaf area index (LAI), leaf area duration (LAD), specific leaf area (SLA), crop growth rate (CGR) and net assimilation rate (NAR) of barley. Nonetheless, bed-sown barley produced the highest biomass due to better crop allometry and soil physical conditions. The highest postharvest soil available P, NH4-N, NO3-N, and K were recorded for zero-tilled barley, while BS followed by CT recorded the lowest nutrient contents. Barley in mungbean-B CS with BS produced the highest biomass, while the lowest biomass production was recorded for barely sown in fallow-B cropping system with ZT. In conclusion, barley sown after mungbean (mungbean-B cropping system) with BS seems a pragmatic choice for improving soil fertility and subsequently soil health.

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Malnutrition is among the biggest threats being faced globally, and Pakistan is among the countries having high malnutrition rate. Pulses grown in Pakistan have lower amounts of micronutrients, especially iron (Fe) in grains compared to developed world. Biofortification,-a process of integrating nutrients into food crops-, provides a sustainable and economic way of increasing minerals/micronutrients' concentration in staple crops. Mungbean fulfills protein needs of large portion of Pakistani population; however, low Fe concentration in grains do not provide sufficient Fe. Therefore, current study was conducted to infer the impact of different Fe levels and application methods on yield, economic returns and grain-Fe concentration of mungbean. Mungbean was sown under four levels of Fe, i.e., 0, 5, 10 and 15 kg Fe ha-1 applied by three methods, i) as basal application (whole at sowing), ii) side dressing (whole at 1 st irrigation) and iii) 50% as basal application + 50% side dressing (regarded as split application). Iron levels and application methods significantly influenced the allometry, yield, economic returns and grain-Fe concentration of mungbean. Split application of 15 kg Fe ha-1 had the highest yield, economic returns and grain-Fe concentration compared to the rest of Fe levels and application methods. Moreover, split application of 15 kg Fe ha-1 proved a quick method to improve the grain-Fe concentration and bioavailability, which will ultimately solve the Fe malnutrition problem of mungbean-consuming population in Pakistan. In conclusion, split application of Fe at 15 kg ha-1 seemed a viable technique to enhance yield, economic returns, grain-Fe concentration and bioavailability of mungbean.

Boron (B) is a vital nutrient equally important for plants and human beings. The native soil fertility of arid and semi-arid regions of Pakistan is very low where chickpea is grown on large scale. In such soils, B deficiency is the leading constraint not only for profitable chickpea production but also lead to low B-enriched chickpea grains. Boron seed coating and inoculation with Btolerant bacteria (BTB) can be effective to improve crop response grown on such soils. Chickpea seeds were coated with different levels of boron (0.0 control; 1.0, 1.5, 2.0, 2.5, and 3.0 g B/kg). Coated and uncoated seeds were divided in two portions and half of these seeds were inoculated with BTB, i.e., Bacillus sp. MN54 while the other half of these seeds were not inoculated with BTB, i.e., Bacillus sp. MN54. Boron seed coating improved seedling growth, nodulation, seed yield, and grain B concentration. Crop response for such characteristics was further augmented with the inoculation of BTB. It was found that seed coating with 1.5 g B kg −1 along with BTB inoculation was the best combination to improve seedling growth, nodulation, and grain yield of chickpea. Higher concentration of B in chickpea seeds was recorded at higher levels of B seed coating (3.0 g B kg −1 soil). Boron seed coating improved grain yield by 9-25% compared with the control treatment. Boron seed coating at lower rates (1.5 g kg −1) along with seed inoculation with BTB Bacillus sp. MN54 seemed a viable option to improve nodulation, growth, yield, and grain B biofortification of chickpea.

Chickpea is widely cultivated on calcareous sandy soils in arid and semi-arid regions of Pakistan; however, widespread boron (B) deficiencies in these soils significantly decreases its productivity. Soil application of B could improve chickpea yield and grain-B concentration. However, optimizing suitable B level is necessary due to a narrow deficiency and toxicity range of B. Nonetheless, the co-application of B-tolerant bacteria (BTB) and synthetic B fertilizer could be helpful in obtaining higher chickpea yields and grain-B concentration. Therefore, this study optimized the level of soil applied B along with BTB, (i.e., Bacillus sp. MN54) to improve growth, yield and grain-B concentrations of chickpea. The B concentrations included in the study were 0.00 (control), 0.25, 0.50, 0.75 and 1.00 mg B kg−1 soil combined with or without Bacillus sp. MN54 inoculation. Soil application of B significantly improved root system, nodulation, yield and grain-B concentration, and Bacillus sp. MN54 inoculation further improved these traits. Moreover, B application at a lower dose (0.25 mg B kg−1 soil) with BTB inoculation recorded the highest improvements in root system (longer roots with more roots’ proliferation), growth, nodulation and grain yield. However, the highest grain-B concentration was recorded under a higher B level (0.75 mg B kg−1 soil) included in the study. Soil application of 0.25 mg B kg−1 with Bacillus sp. MN54 inoculation improved growth and yield-related traits, especially nodule population (81%), number of pods plant−1 (38%), number of grains plant−1 (65%) and grain yield (47%) compared with control treatment. However, the grain-B concentration was higher under the highest B level (1.00 mg kg−1 soil) with Bacillus sp. MN54 inoculation. In conclusion, soil application of 0.25 mg B kg−1 with Bacillus sp. MN54 inoculation is a pragmatic option to improve the root system, nodule population, seedling growth, yield and agronomic grain-B biofortification of chickpea.