The multiplex PCR system developed in this study enabled the detection of all the bacteria under investigation in one reaction tube in a less time- and labor-intensive manner than conventional PCR. These results support the potential clinical use of multiplex PCR for detecting periodontopathic bacteria and for evaluating therapeutic strategies and predicting the prognosis for each subject.
We studied periodontitis severity, oral hygiene, and questionnaire responses at initial visits and compared to results 10 and 5 years previously.We also studied the effectiveness oftreatment as evidenced by improved oral hygiene, and attempted to determine whether the questionnaire provides information useful for treatment.
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