Reviewed briefly is the development of a unique vaccine against hCG, which prevents unwanted pregnancy without blocking ovulation and disturbing menstrual regularity. A recombinant version of this vaccine, amenable to largescale production by industry, in both DNA and protein form has been made. Priming with DNA followed by protein improves further its immunogenicity. Mycobacterium indicus pranii (MiP), a cultivable non-pathogenic mycobacteria has been used in the recombinant vaccine as a potent adjuvant. MiP was developed as an immunotherapeutic vaccine against leprosy. It is approved by the Drugs Controller General of India and USFDA, and has many additional applications in treatment of tuberculosis, curing of ugly ano-genital warts and in prevention and treatment of some cancers.
A simple, accurate, and reliable high-performance liquid chromatography (HPLC) method was developed for determination, separation and estimation of clopyralid active ingredient in its formulation. The active ingredient in formulation was estimated by two methods and cleanup was performed using C18 SPE. Estimation was done by HPLC using 5 µm, C18 column, and mobile phase 0.02 % acetic acid in methanol: acetonitrile (90:10 v/v) and detection at 229 nm. The efficiency of clean up for standard clopyralid was found to be 98.02-98.66% at different concentrations. Overall recoveries for different methods ranged from 98.3-100.1 %. The LOD and LOQ were 0.1 and 0.3 ng respectively. The detector response was linear within concentrations range 0.25-10.0 ng at RSD 1.87%.
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