The “Landscape Of Gut Microbiome - Pan-India Exploration”, or LogMPIE study, is the first large-scale, nationwide record of the Indian gut microbiome. The primary objective of the study was to identify and map the Indian gut microbiome baseline. This observational study was conducted across 14 geographical locations in India. Enrolled subjects were uniformly distributed across geographies (north, east, west and south) and body mass index (obese and non-obese). Furthermore, factors influencing the microbiome, such as age and physical activity, were also considered in the study design. The LogMPIE study recorded data from 1004 eligible subjects and reported 993 unique microorganisms across the Indian microbiome diaspora. The data not only map the Indian gut microbiome baseline but also function as a useful resource to study, analyse and identify signatures characterizing the physiological dispositions of the subjects. Furthermore, they provide insight into the unique features describing the Indian microbiome. The data are open and may be accessed from the European Nucleotide Archive (ENA) portal of the European Bioinformatics Institute (https://www.ebi.ac.uk/ena/data/view/PRJEB25642).
Poly-γ-glutamic acid (PGA) is a homopolyamide, biosynthesized mostly by Bacillus sp. Our study focuses on understanding the genetic differences between the two species of Bacillus for their capability to produce PGA. Genes related to PGA synthesis, regulation, degradation and mannitol utilization of Bacillus subtilis Natto3 (BSN3) were compared with that of B. methylotrophicus IC4 (BMIC4). These strains differed in their genome sizes and average gene lengths. BMIC4 genome size was 4,214,684 bp which was larger than BSN3 comprising of 3,601,055 bp with no plasmid found in either of them. The average gene length of BSN3 and BMIC4 were 843.33 bp and 819.82 bp, respectively with higher number of predicted genes and proteins in BMIC4 (4341 and 4223 respectively). Interestingly, BMIC4 being larger in genome size and gene number, exhibited lesser number of unique pfam results (62) compared to 389 unique pfam of BSN3. Based on 16S rRNA gene sequence, BSN3 and BMIC4 were placed distantly on the phylogenetic tree. Sequence similarity of PGA producing genes ywsC, ywtA and ywtB between BSN3 and BMIC4 was 100%, 100% and 30% respectively. We report the presence of PGA degrading gene pgdS in BMIC4 which is otherwise reportedly absent in various strains of B. methylotrophicus. Sequence variation in the genes may have an impact on the PGA chain length, produced by these strains as BMIC4 produces high molecular weight PGA than BSN3. As B. methylotrophicus is newly discovered species, our comparative study will provide insights on the genomic variability between these two novel PGA producing strains.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.