REMAP ( retrotransposon-microsatellite amplified polymorphism ) is a kind of molecular markers based on retrotransposons .In this study , the genetic diversity of 73 eggplant varieties was examined by REMAP molecular marker .Results showed that 1992 bands were amplified with seven primers designed according to the sequences of MAGGY , BARE-1 and Sukkularc,the ratio of polymorphic bands was 100%, the average number of bands per primer was 284.6. The result of POPGENE analysis indicated that Nei's gene diversity H was 0.3641, Shannon's information index was 0.5424, the highly diverse was displayed obviously.Nei's similarity cofficients among the tested varieties ranged from 0.2115 to 0.9231,The relationships among the varieties were evaluated by UPGMA , showing that the tested eggplant varieties can be classified into eight groups on the molecular level. The REMAP clustering pattern of majority cultivated eggplant did not demonstrate relationship with fruit shape or fruit color.
Molecular marker based on WRKY transcription factor was used to analyze polymorphism in 34 cultivated varieties of eggplant (Solanum melongena L.) with ten primers. The primers were designed based on conserved sequence regions of WRKY genes of tomato and pepper. A total of 1396 bands were successfully amplified by the 10 primer pairs, of these, 1328 bands (95.1%) were polymorphic, the ratios of polymorphism were 71% and 72% for primer GIIF-WSR1 and GIIF-WSR12, the ratio of polymorphism revealed by other 8 primer pairs was 100%. The result showed that the molecular marker based on WRKY transcription factor is high polymorphic in eggplant.
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