A feeding trial was performed for 60 days to determine the dietary lipid requirement of Penaeus vannamei juveniles reared in inland saline water (ISW) of 10 g/L salinity. Six iso-nitrogenous (360 g/kg crude protein) and heterocaloric (15.4-17.6 KJ DE/g) semipurified diets were prepared with graded level of lipid viz., 20, 40, 60, 80, 100 and 120 g/kg (L2-L12 with 20 g/kg incremental level). Two hundred and seventy P. vannamei juveniles (5.51 ± 0.01 g) were randomly allocated (15 shrimps/200 L) into six treatment groups in triplicate following a completely randomized design. The shrimp of L4 and L6 groups exhibited significantly higher per cent weight gain (WG%), specific growth rate and protein efficiency ratio and the lowest feed conversion ratio.The lipid efficiency ratio and hepatopancreatic somatic index were significantly decreased and increased with increasing levels of lipid respectively. The L6, L8 and L10 groups exhibited significantly higher (p < .05) lipase activity. Shrimp of higher dietary lipid fed groups exhibited significantly higher (p < .05) aminotransferase activity than their lower dietary lipid fed counterparts. Based on WG% data, broken-line linear and second-order polynomial regression analysis showed that the optimal dietary lipid requirement of P. vannamei in ISW of 10 g/L salinity was 46.5 and 51.4 g/kg respectively.
A 60-day feeding trial was conducted to evaluate the optimum dietary lipid level in terms of growth performance, digestive enzymes and hemato-biochemical parameters of white-leg shrimp, Penaeus vannamei juveniles in inland ground saline water (IGSW) of 15 ppt salinity. The shrimps (avg. wt., 4.04 ± 0.03 g) were randomly distributed in six groups in triplicates (15 shrimps/tank, 290 L), viz., TCL40 (40 g/kg lipid) , TCL60 (60 g/kg lipid) , TCL80 (80 g/kg lipid), TCL100 (100 g/ kg lipid), TCL120 (120 g/kg lipid) and TCL140 (140 g/kg lipid). Six semi-purified hetero-lipidic (40-140 g lipid/kg), hetero-caloric (376-426 Kcal DE/100 g) and iso-nitrogenous (400 g crude protein/kg) diets were prepared and fed to respective groups four times daily. Weight gain (WG), specific growth rate (SGR), feed conversion ratio (FCR), and protein efficiency ratio (PER) showed higher quadratic relations (R 2 = 0.97, 0.98, 0.94 and 0.87, respectively) to dietary lipid levels (p < 0.05) with enhancing dietary lipid up to 60 g/kg. Whole body moisture and lipid contents exhibited a high linear (R 2 = 0.87 and 0.98) and quadratic (R 2 = 0.88 and 0.98) (p < 0.05) inverse relationship with enhancing dietary lipid levels up to 140 g/kg. Hemolymph hemocyanin, serum total protein and glucose level did not differ significantly (p > 0.05), but the TCL60 group showed significantly (p < 0.05) higher serum cholesterol and triglyceride levels. Serum osmolality, osmoregulatory capacity, and branchial Na + /K + -ATPase activity was similar (p > 0.05) among the groups. Hepatopancreatic lipase and amylase activity significantly (p < 0.05) increased, but trypsin activity did not vary significantly (p > 0.05) among the groups. Second-degree polynomial analysis with WG, SGR and FCR indicated that a 55.80-58.80 g lipid/kg diet is optimum for P. vannamei juveniles in IGSW of 15 ppt salinity.
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