the N-glycome profile in serum is gender and age dependent. This should be taken into consideration in the development of serum glycome markers.
A Morris water maze (MWM) experiment forces experimental animals to swim and learn to find a platform hidden in the water. It is widely used in scientific research to assess the learning and memory of animals. Due to the extensive use of the MWM test, visual experimental protocols are essential for researchers. This manuscript uses the latest studies to introduce the protocol of the MWM test. Alzheimer' Disease (AD) is characterized by a progressive loss of memory and cognitive function. An alternative and complementary treatment used for AD is Manual Acupuncture (MA). To assess the learning and memory ability of AD model mice, the MWM test was conducted. The visible platform trial, hidden platform trial, probe trial, and reversal trial of MWM were used to evaluate spatial learning and memory ability. In the visible platform trial, the swimming speed and escape latency of mice in different groups was not significantly different. In the hidden platform and reversal trials, the AD group showed a long escape latency. The escape latency decreased significantly after the MA treatment. Low platform crossover number and the proportion of time in the SW quadrant in the probe trial increased after the MA treatment (p < 0.05 or p < 0.01). The results of the MWM tests suggest that MA can effectively improve the spatial learning and memory abilities of AD model mice. Rigorous experimental operations provided assurance of the reliability of the results. Video Link The video component of this article can be found at https://www.jove.com/video/60055/ 1. Preparation 1. Purchase 30 male SAMP8 mice and 10 male SAMR1 mice (age: 8 months). 2. House the mice individually in individual ventilation cages at a temperature of 24 ±2 °C and a 12 h dark/light cycle.
Hepatic ischemia reperfusion injury (HIRI) is a clinical condition which may lead to cellular injury and organ dysfunction. The role of nitric oxide (NO) in HIRI is complicated and inconclusive. NO produced by endothelial nitric oxide synthase (eNOS) activation plays a protective role during early HIRI. But eNOS overexpression and the resulting excessive NO bioavailability can aggravate liver injury. NO induced by inducible nitric oxide synthase (iNOS) may have either a protective or a deleterious effect during the early phase of HIRI, but it may protect the liver during late HIRI. Here, we reviewed the latest findings on the role of NO during HIRI: (1) NO exerts a protective effect against HIRI by increasing NO bioavailability, downregulating p53 gene expression, decreasing inflammatory chemokines, reducing ROS via inhibiting the mitochondrial respiratory chain, activating sGC-GTP-cGMP signal pathway to reduce liver cell apoptosis, and regulating hepatic immune functions; (2) eNOS protects against HIRI by increasing NO levels, several eNOS/NO signal pathways (such as Akt-eNOS/NO, AMPK-eNOS/NO and HIF-1α-eNOS/NO) participating in the anti-HIRI process, and inhibiting over-expression of eNOS also protects against HIRI; and (3) the inhibition of iNOS prevents HIRI. Thus, the adverse effects of NO should be avoided, but its positive effect in the clinical treatment of diseases associated with HIRI should be recognized.
Abstract:Water resource allocation is the process of assessing and determining a mechanism on how water should be distributed among different regions, sectors and users. Over the recent decades, the optimal solution for water resource allocation has been explored both in centralised and decentralised mechanisms. Conventional approaches are under central planner suggesting a solution which maximises total welfare to the users. Moving towards the decentralised modelling, the techniques consider individuals as if they act selfishly in their own favour. While central planner provides an efficient solution, it may not be acceptable for some selfish agents. The contrary is true as well in decentralised solution, where the solution lacks efficiency leading to an inefficient usage of provided resources. This paper develops a parallel evolutionary search algorithm to introduce a mechanism in re-distributing the central planner revenue value among the competing agents based on their contribution to the central solution. The result maintains the efficiency and is used as an incentive for calculating a fair revenue for each agent. The framework is demonstrated and discussed to allocate water resources along the Nile river basin, where there exist eleven competing users represented as agents in various sectors with upstream-downstream relationships and different water demands and availability.
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