Objectives
Drug combination has a promising and potential development prospect in the treatment of various cancers. The objective of this study is to investigate the synergistic mechanisms of polyphyllin
VII
(
PVII
) and formosanin C (
FC
) in lung cancer.
Materials and methods
The combination of
FC
and
PVII
influenced on the apoptosis, autophagy, and the relative signalling pathways were analysed in lung cancer cells.
Results
The combination of
FC
and
PVII
demonstrated a concentration‐ dependent growth inhibition in human lung cancer cells. The combination index (
CI
) obtained from four lung cancer cells was smaller than 1. This synergistic antitumour effect was based on the increase of their single proapoptotic effect but inhibiting
FC
‐induced autophagy in
NCI
‐H460 cells.
FC
and
PVII
activated proapoptotic elements like cleaved‐caspase‐3, ‐8, and ‐9 to induce Beclin1 cleaved into Beclin1‐C which suppressed
FC
‐triggered autophagy and enhanced apoptosis.
Conclusions
Formosanin C and
PVII
showed a synergistic antitumour effect on lung cancer cells. The findings would provide the foundation for the use of combination drugs in the future.
Pseudomonas sp. strain KENGFT3 inhibits the growth of Phytophthora infestans and is a potentially useful biopesticide for plant diseases, including potato late blight. We sequenced the 6.2-Mbp genome of this strain and assembled it into a single scaffold with 9 contigs. KENGFT3 is related to previously sequenced strains of P. fluorescens.
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