Cellulase is one of the important groups of enzyme for many industrial applications. It is applied in various processes of food industries, paper and pulp industry, fabric industry, medicine and pharma industry, agriculture industries etc. Microorganisms are among the most potential source for large scale production of this enzyme. The current study is aimed to isolate microorganisms which are capable of producing cellulase and to determine its efficiency for production of cellulase with different raw materials. For this study, bacteria microorganisms were isolated on CMC containing media. Screening was done using congo red (0.1%) and NaCl (1%) solution. Among various isolated species, Bacillus amyloliquefaciens was identified as the most potential strain and hence selected for further study, various raw materials which are rich in cellulose were used for cellulase production. These raw materials were sugarcane bagasse, paper pulp, molasses, orange peel, wheat bran, cassava waste, tea waste and agriculture waste. Cellulase activity was determined by using dinitrosalicylic acid (DNSA) method. Results of the study have shown that among all the raw materials, sugarcane bagasse, molasses and paper pulp were found as the most potential sources for enzyme production. DNSA have shown 0.97 IU/mL, 0.98IU/mL and 0.88 IU/mL of enzyme activity respectively for all the three raw materials. Results have also shown that agriculture waste alone is not found suitable for production of cellulase as it has shown only 0.32 IU/mL of enzyme activity. From the entire study, it was concluded that for large scale production of cellulase, sugarcane bagasse and molasses are the best sources using Bacillus amyloliquefaciens.
Fermentation technology plays a very important role in the field of Biotechnology. Production of various enzymes, antibiotics, single cell proteins, various food products, etc is carried out in a vessel called as the bioreactor which is the seed of fermentation technology. The project includes study of Pectinase production using submerged fermentation and various parameters like different substrate, different strains and fermentation time to obtain the maximum yield of Pectinase. To achieve this various research is being conducted. Bacillus subtilis species was being used in this work for the production of Pectinase.
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