Methylmercury (MeHg) is the most biologically available and toxic form of mercury, and can act as a powerful teratogen, neurotoxin and endocrine disruptor in vertebrates. However, mechanisms of endocrine impairment and net effects on demography of biota are poorly understood. Here, we report that experimental exposure of an aquatic bird over 3 years to environmentally relevant dietary MeHg concentrations (0.05 -0.3 ppm wet weight) resulted in dose-related increases in male -male pairing behaviour (to 55% of males), and decreases in egg productivity (to 30%). Dosed males showed decreased rates of key courtship behaviours, and were approached less by courting females in comparison to control males. Within dosed groups, homosexual males showed a similar reduction when compared with dosed heterosexual males. We found an average 35 per cent decrease in fledgling production in highdose birds over the study duration. These results are of interest because (i) MeHg exposure is experimentally tied to demographically important reproductive deficits, (ii) these effects were found at low, chronic exposure levels commonly experienced by wildlife, and (iii) effects on reproductive behaviour and sexual preference mediated by endocrine disruption represent a novel and probably under-reported mechanism by which contaminants may influence wild populations of birds.

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Asian elephants (Elephas maximus) are threatened primarily by habitat loss and human-elephant conflict. In addition to establishing protected areas and corridors for wildlife, empowering farmers to protect their crops is crucial for Asian elephant conservation [1,2]. Elephants can habituate to artificial deterrents, hence natural biological alternatives are of great interest [2,3]. African elephants (Loxodonta africana) avoid African honey bees (Apis mellifera scutellata), inspiring 'beehive fences' as a successful means of small-scale crop protection [4,5]. Here, we used a recording of a disturbed hive of cavity-dwelling Asian honey bees (Apis cerana indica) and conducted sound playbacks to 120 wild elephants in 28 different groups resting under trees in Uda Walawe National Park in Sri Lanka. Elephants responded by moving significantly further away from their resting site in bee playback trials compared to controls. Elephants also increased vocalization rates, as well as investigative and reassurance behaviours in response to bee sounds, but did not display dusting or headshaking behaviour.

Toxin emissions and legacies are major global issues affecting many species through, among other effects, endocrine disruption and reproductive impairment. Assessment of toxin risk to wildlife focuses mostly on offspring-related metrics, while the lack of breeding initiation or early breeding failure has received less attention. We tested whether exposure to methyl mercury (MeHg) results in early breeding failure and reduced number of breeding birds using observational and experimental data. We used 21 years of numbers of breeding pairs of colonially breeding wild Great Egrets (Ardea alba) in response to annual and geographical variation upon exposure to environmental MeHg. After controlling for food availability, we found a strong negative association between MeHg exposure and the number of breeding Great Egrets. We report reductions of >50% in breeding numbers under exposure levels otherwise associated with <20% reduction in post-egg-laying breeding success. Experimental exposure of White Ibises (Eudocimus albus) to MeHg also caused early breeding failure and a ∼20% reduction in breeding numbers at environmentally relevant exposures. The demographic consequences of reductions in breeding pairs are additive to known and typically studied impairments in postlaying reproductive success. Net demographic effects of exposure to endocrine disruptors may often be strongly underestimated if early breeding failure is not measured.