Anahtar kelimeler: Alkalifilik, prokaryotik çeşitlilik, Salda Gölü, yeni nesil dizileme ABSTRACT: In our study, water samples were taken from Salda Lake and the pH and salinity data for the sample were determined. After that, samples were filtered and DNA extraction was performed. The obtained DNA was
Toluene is an aromatic hydrocarbon and spreads to the environment by the release of petroleum products, agricultural and industrial activities and toluene can cause serious social and health problems. Since the removal in toluene is considered an important environmental issue, there is an increasing interest of toluene biodegradation. In this comprehensive study, novel toluene degrading bacteria were isolated and identified both from municipal and industrial wastewater treatment plants. Isolates' toluene tolerance, enzyme production, benzene, ethyl benzene, xylene and phenol degradation potentials and biofilm formation were examined. Totally 109 bacterial isolates were obtained and most of them were determined as belong to Stenotrophomonas, Acinetobacter and Pseudomonas species.Isolates' toluene tolerance, enzyme production, benzene, ethyl benzene, xylene and phenol degradation potentials and biofilm formation were examined. Most of isolates showed lipase, DNase and protease activity. Most of isolates showed growth in 300 mg/l -1 concentrations of benzene, ethyl benzene, xylene and phenol. Strains E4T16, 3AT2 and 3ET5 showed maximum growth in high toluene concentrations and also strain E4T16 showed growth up to 2100mg/l -1 toluene. Examination of biofilm formation at different toluene concentrations at the end of the 96 hours incubation revealed that strains E4T16, 4ET21 and 3ET5 had highly biofilm production potential. In conclusion, these bacteria and results could be considered as a powerful new approach for the removal of hydrocarbons from wastewater.
Van Lake, located in Eastern Anatolia Region, is the largest lake in Turkey. This lake features alkaline and saline soda lake. Since there are few studies focused on microbial diversity of alkaline environments, it is very important to reveal the diversity of microorganisms of Van Lake because of the wide range of biotechnological uses of these microorganisms and their ecological roles in lake ecosystem. Next generation sequencing (NGS) allow us to sequence DNA and RNA much more quickly and cheaply than the previously used Sanger sequencing for the rapid analysis of the composition and diversity of microbial communities in several habitats. We applied the high throughput techniques of NGS to the metagenomics study of Van Lake, by assessing its PCR amplicon of 16S rDNA sequences (V3-V4 regions). The analyses revealed Proteobacteria, Actinobacteria and Verrucomicrobia phyla were most abundant. Archaea members were represented by Euryarchaeota phylum. Results concluded that Bacteria domain were dominant in Van Lake.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.