The aim of the present work was to optimize the process parameters of the nano spray drying technique for the formulation of benzydamine-loaded casein nanoparticles and to investigate the effect of some process variables on the structural and morphological characteristics and release behavior. The obtained particles were characterized in terms of particle size and size distribution, surface morphology, production yield and encapsulation efficiency, drug-polymer compatibility, etc., using dynamic light scattering, scanning electron microscopy, differential scanning calorimetry, and Fourier transformed infrared spectroscopy. Production yields of the blank nanoparticles were significantly influenced by the concentration of both casein and the crosslinking agent. The formulated drug-loaded nanoparticles had an average particle size of 135.9 nm to 994.2 nm. Drug loading varied from 16.02% to 57.41% and the encapsulation efficiency was in the range 34.61% to 78.82%. Our study has demonstrated that all the investigated parameters depended greatly on the polymer/drug ratio and the drug release study confirmed the feasibility of the developed nanocarriers for prolonged delivery of benzydamine.

The aim of this study was to develop casein-based nanoscale carriers as a potential delivery system for daunorubicin, as a pH-responsive targeting tool for acute lymphocytic leukemia. A coacervation technique followed by nano spray-drying was used for the preparation of drug-loaded casein nanoparticles. Four batches of drug-loaded formulations were developed at varied drug–polymer ratios using a simple coacervation technique followed by spray-drying. They were further characterized using scanning electron microscopy, dynamic light scattering, FTIR spectroscopy, XRD diffractometry, and differential scanning calorimetry. Drug release was investigated in different media (pH 5 and 7.4). The cytotoxicity of the daunorubicin-loaded nanoparticles was compared to that of the pure drug. The influence of the polymer-to-drug ratio on the nanoparticles’ properties such as their particle size, surface morphology, production yield, drug loading, entrapment efficiency, and drug release behavior was studied. Furthermore, the cytotoxicity of the drug-loaded nanoparticles was investigated confirming their potential as carriers for daunorubicin delivery.

The aim of the present research is the preparation of chitosan nanoparticles intended for Benzydamine (BZ) delivery. The method of ionotropic gelation is applied for the nanoparticle's formation. The crosslinking is performed by NaTPP. Dynamic light scattering analysis shows that particle's sizes are in nano‐scale and can be varied by changing the polymer and crosslinker concentrations. The yield of the gelation varies between 23% and 69%. The BZ loading efficiency differs in the range from 10% to 24.3%. Differential scanning calorimetry is applied for investigating the BZ state. It proved that the BZ changes its physical state from crystal to amorphous. The in vitro drug release profile along with kinetics and mechanism of release from the nano‐spheres are studied under simulated physiological conditions for different incubation periods. The release rate can be changed by varying the chitosan and NaTPP concentrations.

The incessant need for the elimination of pathogenic viruses and multi-drug resistant bacteria has been a critical issue during recent decades, and requires the creation of new antimicrobial materials. Our study describes the production of silver-modified anodic alumina substrates by two methods, and estimation of their bactericidal activity. Aluminum oxide coatings were obtained via an anodization process of low-purity aluminum in an acidic bath for different time periods. The realization of silver infiltration into the pores of the alumina layers was carried out employing two different routes—electrochemical deposition, and in situ thermal reduction. The obtained films were characterized using scanning electron microscopy (SEM). Changes in the surface morphology and thickness of the initial oxide structures after hot water sealing procedure were observed. The presence of silver inside the pores of the alumina layers was also assessed. It was found that silver electrodeposition resulted in greater surface saturation. Large silver accumulations were observed on the thinner anodic films which experienced electroplating for longer time periods. Finally, the antibacterial activity of the modified alumina structures against Gram-negative (Escherichia coli) and Gram-positive (Bacillus cereus) bacteria was evaluated. The results demonstrate that silver deposits acquired by the electrochemical technique improve the bactericidal efficiency of the anodic aluminum oxide (AAO) layers. On the contrary, alumina structures with chemically embedded Ag particles did not show significant antibacterial properties. Overall, the present studies demonstrate that biological activity of silver-doped AAO films depends on the techniques used for their modification.

The present study reports a specific method for preparation of silver-modified anodic alumina substrates intended for biomaterial applications. Al2O3 coatings were obtained by anodization of technically pure aluminum alloy in sulfuric acid electrolyte. Silver deposition into the pores of the anodic structures was carried out employing in situ thermal reduction for different time periods. The obtained coatings were characterized using scanning electron microscopy (SEM), potentiodynamic scanning after 168 h in 3.5% NaCl solution and bioassays with human fibroblast and NIH/3T3 cell lines. The modified alumina substrates demonstrated better biocompatibility compared to the control anodic Al2O3 pads indicated by increased percent cell survival following in vitro culture with human and mouse fibroblasts. The Ag-deposition time did not affect considerably the biocompatibility of the investigated anodic layers. SEM analyses indicated that mouse NIH/3T3 cells and human fibroblasts adhere to the silver-coated alumina substrates retaining normal morphology and ability to form cell monolayer. Therefore, the present studies demonstrate that silver coating of anodic alumina substrates improves their biocompatibility and their eventual biomedical application.

The aim of this study was to develop nanosized-casein carriers by the method of spray drying and to evaluate their potential as a tool for delivery of daunorubicin in the treatment of acute lymphocytic leukaemia (ALL). Full 3 2 factorial design was applied to evaluate the optimal production parameters (concentration of the polymer and the crosslinker) for the preparation of blank casein nanoparticles. Nine batches of unloaded particles were developed and characterized in terms of particle size, size distribution, surface morphology and compatibility between the drug and the polymer. Based on an optimized "placebo" model of casein nanostructures, four batches of daunorubicin-loaded particles were synthesized at varied drug-polymer ratios. The obtained structures have average particle size within the range 127 to 167 nm, and encapsulation efficiency was between 42.8% and 61.8%. Delayed drug release was demonstrated, which correlates with the results of the cytotoxicity study on lymphoblast cells.

No abstract

Production of nanoparticles by nano spray drying is strongly influenced by the process parameters -polymer concentration, solubilizing agent concentration, inlet temperature, pumping speed, spray rate, as well as the spray mesh size.