CrossFit (CF) is a popular and rapidly expanding training program in Greece and worldwide. However, there is a lack of scientific evidence on the risk of musculoskeletal injuries related to CF in the Greek population. A self-administered survey of 1224 Greek CF practitioners aged 18 to 59 was conducted and analyzed using the Statistical Package for Social Sciences (SPSS) software. The highest percentage of the participants (34%) practiced 5 days per week for 60 min (42.2%) and had 2 days per week of rest (41.7%). A total of 273 individuals (23%) participated in CF competitions and 948 (77%) did not. The results showed that the most common injuries were muscle injuries (51.3%), followed by tendinopathies (49.6%) and joint injuries (26.6%). The shoulders (56.6%; n = 303), knees (31.8%; n = 170), and lumbar spine (33.1%; n = 177) were the most commonly injured locations. The logistic regression model showed that participation in competitions (p = 0.001), rest per week (p = 0.01), duration of training per session (p = 0.001), and frequency of training per week (p = 0.03) were statistically significant factors for injury. Training level was not a statistically significant factor for injury (p = 0.43). As CF continues to gain popularity on a global scale and the number of athletes gradually increases, it is important to monitor the safety of practitioners. Clinicians should consider participation in competitions, rest, training duration, and frequency in order to make CF safer.
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