No abstract

Illegal hunting has been a major threat for the survival of wildlife fauna, including the three crocodile species that India harbours: Crocodylus palustris, Crocodylus porosus and Gavialis gangeticus. Although law prevents trade on these species, illicit hunting for trade continues to threaten the survival of these endangered species; conservation strategies therefore require a rapid molecular identification technique for Indian crocodiles. A multiplex polymerase chain reaction (PCR) assay with species-specific primers, considered as one of the most effective molecular techniques, is described herein. The primers were designed to yield species-specific sized amplicons. The assay discriminates the three Indian crocodile species unambiguously within a short time period using only simple agarose gel electrophoresis. We recommend this multiplex PCR assay to be used in the identification of Indian crocodile species.

Comprehensive hematologic and biochemical analyses were conducted on blood from 23 male and 31 female clinically stable captive mugger crocodiles (Crocodylus palustris). Erythrocyte mean corpuscular volume (MCV), potassium, cholesterol, and calcium concentrations were significantly greater in juvenile males than in juvenile females, but no significant differences were determined between parameters of subadult males and subadult females. The mean WBC count and mean heterophil count were significantly higher in adult males than in adult females. Mean uric acid concentration was significantly greater in adult females than in males. Mean erythrocyte count was significantly higher in adults than in juveniles. Adult mean WBC and lymphocyte counts were significantly lower than those of both juveniles and subadults. Subadults had significantly lower mean eosinophil counts than both adults and juveniles. Subadults had significantly lower mean alkaline phosphatase activities than juveniles, whereas the adults had significantly lower aspartate aminotransferase and alanine aminotransferase activities than other groups. Lactate dehydrogenase activities were significantly lower for subadults than for juveniles and adults. Cholesterol concentrations were significantly higher for subadults and juveniles compared with adults. Triglyceride concentration was significantly lower for subadults and highest for juveniles. Glucose concentrations were significantly higher for adults. Blood urea nitrogen was significantly lower for subadults than for both adults and juveniles. Uric acid concentrations were significantly higher for juveniles than for the subadults and adults. The subadult animals also had a significantly lower potassium concentration. The results obtained were then compared with known values for other crocodilian species.

Reproductive biology of Aspideretes gangeticus was studied between 1986 and 2001. Clutchsize averaged 17.9 eggs and ranged between six to thirty-fi ve eggs. Egg length averaged 30.6 mm,egg width averaged 30.22 mm, and egg weight averaged 16.85 g. Clutch volume averaged 253.75ml. No signifi cant difference was observed in clutch size between dry and wet seasons. Of the variousincubation protocols tested, one that involved transitional temperatures of 28º – 31º C, to chilling at15º – 18º C, and then 23º – 26º C resulted in the highest hatching success. Aspideretes gangeticusexhibit two forms of development arrest during incubation, embryonic diapause early in incubation andembryonic aestivation in the latter trimester of incubation. The two Aspideretes gangeticus femalesthat produced clutches for the current study produced eggs with a high fertility percentage throughoutthe fi fteen years for which they stored sperm.

A study was conducted to examine the population estimate and spatial distribution of Mugger Crocodile in the southern stretch of river Cauvery, Hosur Forest Division from February 2019 to May 2019. In total, 53 basking sites and 45 Muggers were encountered by direct sighting in the 24-km river stretch using daytime ground based survey approach. N-mixture models estimated an average Mugger density of 2.05 individuals per kilometre for daytime survey. A night spotlight survey across the seven segments of river stretch was also conducted which yielded direct sightings of 54 Muggers with an average encounter rate of 2.25 individuals per kilometre. Two crocodile nests with hatched egg shells were also observed on the sand banks of the river. We concluded that a potential healthy and breeding population of Mugger inhabits the studied stretch of the river. Multiple corresponding analysis was also performed, which demonstrated that Mugger responds to sandy banks alongside deep water pools for basking in contrast to river segments with shallow depth and dense riparian cover.